Alaabta

Monogalactopyranosides of fluorescein iyo fluorescein methyl ester: isku-dhafka, enzymatic hydrolysis by biotnylated β-galactosidase, iyo go'aaminta isku-dhafka fidinta tarjumaadda

Fluorescein monoglycosides (d-galactopyranoside (FMG) iyo d-glucopyranoside) iyo methyl ester (MFMG) ayaa laga diyaariyey acetobromoglucose / galactose iyo fluorescein methyl ester wax soo saar wanaagsan.Tijaabooyin hydrolysis enzymatic (iyadoo la adeegsanayo biotinylated β-galactosidase) ee ka-soo-saarka galacto ayaa la sameeyay waxaana la xisaabiyay cabirrada kinetic.Kordhinta 15-20 jeer ee xoojinta iftiinka fluorescence ayaa la arkay inta lagu jiro qulqulka biyaha.Korodhka toosan ee fluorescence ayaa lagu xusay wakhti gaaban iyo xooga hoose ee substrate, taasoo ka dhigaysa xeryahooda faa'iido leh oo xasaasi u ah galactosidases.Baaxadda qiimaha joogtada ah ee Michaelis-Menten (Km) ee MFMG ayaa ka sarreeya kan FMG oo soo jeedinaya isbeddel qaabaysan oo suurtagal ah ee substrate-ka fluorogenic.Qiimaha km ee β-Gal biotinylated ee leh FMG wuu ka hooseeyaa kan enzyme-ga hooyo.U fiirsashadani waxay muujinaysaa xidhidhnimada substrate-ka sare ee enzyme-ka biotinylated marka la barbar dhigo enzyme-ga hooyo.Isku-duwayaasha faafinta tarjumaadda ayaa la cabbiray, labada nooc ee fluorogenic substrates iyo labadaba badeecadaha, iyadoo la adeegsanayo spectroscopy correlation fluorescence.Isku-duwayaasha faafinta tarjumaadda ee substrate-ka fluorogenic iyo alaabada enzymatic hydrolysis ayaa lagu qiyaasay inay la mid yihiin, inta u dhaxaysa 3.5-4.5 × 10-10 m2 s-1.Markaa kor u qaadis ama dib u dhac ku yimaadda kinetics enzymatic sababtoo ah kala duwanaanshaha dhaqdhaqaaqa tarjumaada substrate-ka iyo badeecada maaha mid muuqata.