Hidde-side β-glucosidase novel, bgl1G5, ayaa laga sameeyay Phalophora sp.G5 oo si guul leh loogu muujiyey Pichia wadaadada.Falanqaynta isku xigxiga waxay muujisay in hidde-socodku ka kooban yahay 1,431-bp qaab akhrin furan oo codeeynaya borotiin ah 476 amino acids.Isku xigxiga amino acid ee bgl1G5 ayaa muujisay aqoonsi sare oo ah 85% oo leh β-glucosidase lagu gartaa oo ka yimid Humicola grisea ee qoyska glycoside hydrolase 1. Marka la barbardhigo fangaska kale ee fangas, Bgl1G5 waxay muujisay waxqabad la mid ah pH 6.0 iyo 50 ° C waxayna ahayd mid deggan. pH 5.0-9.0.Waxaa intaa dheer, Bgl1G5 waxay soo bandhigtay heerkulbeeg wanaagsan 50 °C (6 h nus nolosha) iyo hawlo gaar ah oo sarreeya (54.9 U mg-1).Qiimaha ugu sarreeya ee K m iyo V ee p-nitrophenyl β-D-glucopyranoside (pNPG) waxay ahaayeen 0.33 mM iyo 103.1 μmol min-1 mg-1, siday u kala horreeyaan.Qiimaynta gaarka ah ee substrate-ka ayaa muujisay in Bgl1G5 uu aad ugu firfircoonaa pNPG, daciifna p-nitrophenyl β-D-cellobioside (pNPC) iyo p-nitrophenyl-β-D-galactopyranoside (ONPG), oo aan wax dhaqdhaqaaq ah ku lahayn cellobiose.
