Alaabta

Daraasaddan waxaa loo sameeyay si loo baaro saameynta faafinta ee peptides soy khudaarta ee unugyada asliga ah ee qaangaarka ah (ASCs) maqnaanshaha serum iyo hababka suurtagalka ah ee ficilka.Kordhinta unugyada tarma ee mesenchymal-ka ee unugyada adipose-ka (ADSCs) iyo unugyada dhiigga ee xudunta ka soo jeeda unugyada asliga ah (CB-MSCs) oo lagu daaweeyay peptides soy ayaa la ogaaday inay si weyn u korodhay 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide iyo Click-iT 5-ethynyl-2'-deoxyuridine socodka cytometry.Intaa waxaa dheer, peptides soy waxay keentay fosforyaal-socod ah oo ah p44/42 MAPK (ERK), bartilmaameedka naasleyda ee rapamycin (mTOR), p70 S6 kinase, S6 ribosomal protein (S6RP) iyo factor initiation factor 4E (eIF4E) ee ADSCs.Intaa waxaa dheer, falanqaynta tirada ee cytokines ayaa shaaca ka qaaday in wax soo saarka isbeddelka isbeddelka koritaanka-beta1 (TGF-β1), korriinka kobaca endothelial ee xididdada dhiigga iyo interleukin-6 ay si weyn u korodhay iyada oo laga jawaabayo daaweynta peptides soy ee labadaba ADSCs iyo CB-MSCs.Sidoo kale, fosforyaalka soy peptide-ku-soo-baxa ee dariiqa ERK/mTOR/S6RP/eIF4E ayaa la xannibay iyada oo laga jawaabayo daawaynta hore ee PD98059, oo ah xakameyn ERK gaar ah.Waxaa intaa dheer, xannibaadda TGF-β1 iyada oo loo marayo PD98059 pretreatment iyo hoos u dhac isdaba joog ah ee faafinta ADSC ayaa shaaca ka qaaday in TGF-β1 ay keento fosforyaalka mTOR / S6RP / eIF4E.Isku soo wada duuboo, natiijooyinka daraasaddan ayaa tilmaamaya in wax soo saarka ERK-ku-tiirsan ee TGF-β1 uu door muhiim ah ka ciyaaro kororka soy peptide-ku keeno ADSC-yada hoos yimaada xaaladaha serum-free. Xuquuqda daabacaadda © 2012 Elsevier Inc.