PMSF Cas: 329-98-6 98.0% budada crystalline cad Phenylmethanesulfonyl fluoride (PMSF)
| Lambarka buugaagta | XD90250 |
| Magaca Alaabta | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Qaanuunka molecular | C7H7FO2S |
| Miisaanka Molecular | 174.1927 |
| Faahfaahinta Kaydinta | Ambient |
| Xeerka Tarifka La Iswaafajiyay | 29049900 |
Tilmaamaha Alaabta
| Qiimayn | ≥98.0% HPLC |
| Muuqashada | budo crystalline cad |
PMSF waa serine/cysteine protease inhibitor aan la beddeli karin oo caadi ahaan loo isticmaalo diyaarinta unugyada unugyada.
Daraasadaha gudaha ee vitro: PMSF (2 mM) waxay joojisay uruurinta fosfooraska inositol-kicin-kicinta ee karbachol kaliya 15%-19% joogitaanka Li+.Joojinta beddelka phosphoinotide ee PMSF waxaa sabab u ah hal ama dhowr tillaabo kadib burburka fosfoositide [1].PMSF waxay joojisaa acylation of inositol hadhaaga dhexe ee GPI ee dhiiga T. brucei.PMSF waxay joojisaa samaynta glycolipid C laakiin ma aha dib u habeynta aashitada dufanka leh ee vitro.PMSF waxay joojisaa isku darka GPI acylation iyo ethanolamine fosfatase ee trypanosomes procyclic, laakiin kuma jiraan unugyada Hela [2].
Daraasadaha vivo: PMSF (0.1 mL / 10 g b.wt, ip) waxay soo saartay antinociception, sida lagu muujiyay korodhka jawaab celinta qiyaasta ee% MPE ee qiimeynta daahitaanka dabada, laakiin waxay ku guuldareysatay inay soo saarto xakameyn cad oo ka jawaab celinaysa dhaqdhaqaaqa dhaqdhaqaaqa.Jiirarka helay cirbadeynta intraperitoneal ee PMSF waxay soo bandhigtay saameynaha cannabinoid oo ay ku jiraan antinociception, hypothermia iyo dhaqdhaqaaq la'aanta leh ED50 qiimaha 86, 224 iyo 206 mg / kg, siday u kala horreeyaan.PMSF (30 mg / kg) pretreatment waxay kor u qaadaysaa saamaynta anandamide ee jawaabaha dabada-flick (antinociception), dhaqdhaqaaqa lugaha iyo dhaqdhaqaaqa 5-laab, 10-laab iyo 8-laab, siday u kala horreeyaan [3].
Tijaabooyin xayawaan ah: Jiirarka ICR ee lab ah oo miisaankoodu yahay 18 ilaa 25 g ayaa loo adeegsaday baaritaanka.PMSF waxaa lagu milmay saliid sisinta waxaana lagu maamulay intraperitoneal mug dhan 0.1 mL/10 g b.wt.Had iyo jeer maamul PMSF 10 daqiiqo ka hor anandamide-ka xididada ama duritaanka baabuurta.Jiirarka ayaa habeenki la soo galiyay qolka qiimaynta iyada oo aan la joojin cunto iyo biyo.Iyadoo la raacayo anandamide xididada ama maamulka gaadiidka, xayawaan kasta ayaa la qiimeeyay sida soo socota: 5 daqiiqo jawaabaha daah-fuck (antinociceptive) iyo 5 ilaa 15 daqiiqo dhaqdhaqaaqa iskiis ah (mootada);ama 5 daqiiqo heerkulka xudunta (malawadka) iyo giraanta Dhaqdhaqaaqa (catalepsy) 5 ilaa 10 daqiiqo.
