Alaabta

Acridine ester (NSP-SA-NHS) Cas199293-83-9 iyo xeryahooda la xidhiidha ayaa ah calaamado aad u faa'iido leh oo kimiluminescent ah kuwaas oo degenaanshahooda, firfircoonidooda iyo dareenkooda ay dhaafeen qaar ka mid ah radioisotopes.Esters Acridine waxay ka falcelin kartaa borotiinno ka kooban kooxaha amino-yada aasaasiga ah.Xaaladaha aasaasiga ah, NHS waxa lagu beddelaa koox bixisa, iyo borotiinku waxa ay samaysaa xidhid amide deggan oo leh acridine ester.Ka dib markii falcelinta la dhammeeyo, milixda acridinium ee xad-dhaafka ah ayaa laga saaray iyada oo loo marayo tiir macaan.

Borotiinnada lagu calaamadeeyay Acridine uma baahna kicinta enzymatic si ay u iftiimiso iftiinka joogitaanka hydrogen peroxide ee alkaline.Mabda'a gaarka ah ee iftiiminta iftiinka ayaa ah in xalka alkaline hydrogen peroxide, acridine ester lagu weeraray hydrogen peroxide ions si ay u abuurto dioxyethane aan degganeyn oo kacsan, taas oo sii xumaanaysa CO2 iyo acridone elektaroonik ah oo faraxsan.Marka akridone-ku ku soo noqdo gobolka dhulka, waxa ay soo saartaa sawir-qaade leh dhererka dhererka nuugista ugu badnaan ee 430 nm.Habka iftiinka iftiinka waa mid aad u gaaban (habsocodku wuxuu qaadanayaa wax ka yar 2 ilbiriqsi), nidaamka kicinta waa inuu ku daraa sawir-qaade gudaha ah iyo qalabka sawir-qaade;Intaa waxaa dheer, alaabtani waxay sidoo kale isticmaali kartaa akhristaha microplate-ka badan ee shaqeynaya oo ku qalabaysan autosampler si loo ururiyo xogta iftiinka.Borotiinno, peptides, unugyada difaaca jirka, iyo asiidhyada nucleic ayaa dhamaantood lagu calaamadayn karaa alaabtan.Esters Acridine waxay si dhakhso ah u sii daysaa iftiin hoos yimaada kicinta hydrogen peroxide ee alkaline, markaa xeryahooda calaamadeysan waxaa lagu ogaan karaa ururinta sawir-qaadista.

Alaabtan waxaa inta badan loo isticmaalaa: chemiluminescence iyo immunoassay, falanqaynta reseptor, nucleic acid iyo peptide ogaanshaha iyo cilmi kale.