Alaabta

1.Optically active D-2-hydroxybutanoate waa dhex dhexaad dhisme muhiim u ah dawooyinka iyo poly (2-hydroxybutanoate).Xallinta Kinetic ee jinsiyadda 2-hydroxybutanoate waxay noqon kartaa beddel cagaaran oo la jecel yahay oo loogu talagalay soosaarka D-2-hydroxybutanoate.Shaqadan, D-2-hydroxybutanoate oo ah feejignaan sare (0.197 M) iyo xad-dhaaf ah enantiomeric (99.1%) waxaa soo saaray NAD-madax-bannaan ee L-lactate dehydrogenase (L-iLDH) oo ka kooban biocatalyst.2-Oxobutanoate, dhexdhexaad kale oo muhiim ah, ayaa la soo saaray si aad u sarreeya (0.193 M).Iyadoo la adeegsanayo habka sarifka ion ee fudud oo leh macroporous anion resin sarrifka D301, D-2-hydroxybutanoate ayaa laga soocay nidaamka biotransformation oo leh soo kabasho sare oo ah 84.7%.

2.Asymmetric synthesis of amino acid aan dabiici ahayn waxaa muujiyay omega-transaminase ka Vibrio fluvialis JS17.L-2-Aminobutyric acid waxaa laga soo saaray 2-oxobutyric acid iyo benzylamine oo leh xad-dhaaf enantiomeric oo ka sarreeya 99%.Dareen-celinta waxay muujisay xannibaad ba'an oo badeecada ah by benzaldehyde, kaas oo laga adkaaday iyadoo la adeegsanayo habka falcelinta laba-geesoodka ah si looga saaro badeecada xannibaadda ee wejiga aqueous.Dareen-celinta biphasic ee caadiga ah (50 mM 2-oxobutyric acid, 70 mM benzylamine iyo 2.64 U / ml enzyme la nadiifiyey) iyadoo la isticmaalayo hexane sida soosaar, beddelka 2-oxobutyric acid wuxuu gaadhay 96% 5 h halka kaliya 39% beddelaad la helay iyada oo aan la helin. soo saarista alaabta.