Alaabta

spectroscopy Infrared spectroscopy ayaa loo isticmaalay in lagu sawiro isdhexgalka substrate-protein: isbeddelada is-beddelka ee reticulum sarcoplasmic Ca (2+) -ATPase oo ku xiran nucleotide xidhitaanka iyo fosforyaalka ATPase ayaa la kormeeray iyadoo la adeegsanayo substrate ATP iyo ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, iyo inosine 5'-triphosphate), kuwaas oo wax laga beddelay kooxo gaar ah oo shaqeynaya ee substrate-ka.Wax ka beddelka 2'-OH, 3'-OH, iyo kooxda amino ee adenine waxay hoos u dhigtaa xajmiga isbeddelka is-beddelka is-beddelka ee ATPase, oo leh saameyn xoog leh oo lagu arkay labada dambe.Tani waxay muujinaysaa dareenka qaabdhismeedka nucleotide-ATPase kakanaanta ee isdhexgalka shakhsi ahaaneed ee u dhexeeya nucleotide iyo ATPase.Dhammaan kooxaha la darsay waxay muhiim u yihiin isku-xidhka iyo isdhexgalka kooxda ligand ee la siiyay ATPase waxay ku xiran tahay isdhexgalka kooxaha kale ee ligand.Fosforyaalka ATPase waxaa lagu arkay ITP iyo 2'-deoxy-ATP, laakiin ma ahan 3'-deoxy-ATP.Ma jiro xiriir toos ah oo u dhexeeya heerka isbeddelka is-beddelka ee ku xiran nucleotide iyo heerka fosforyaalka oo muujinaya in baaxadda buuxda ee isbeddelka is-beddelka ee ATP-ku aanu ahayn mid khasab ah fosforyaalka.Sida lagu arkay isku-dhafka nucleotide-ATPase, isku-dhafka fosforyaalka ugu horreeya ee ATPase dhexdhexaadinta E1PCa (2) ayaa sidoo kale ku xiran nucleotide, taas oo muujinaysa in dawladaha ATPase ay leeyihiin qaab isku mid ah oo ka yar sidii hore loo filayay.