Alaabta

Si gaar ah loo qiyaaso dhowr metabolite ee 5-fluorouracil (5-FU) iyo laba monophosphate nucleotides, waxaan sameynay qaab asal ah oo ku saleysan dareeraha chromatography-tandem mass spectrometry (LC-MS/MS).Qiimayntani waxay ogolaatay go'aaminta: (i) wax soo saarka intracellular ee 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP) laga bilaabo 5-FU ama 5-fluoro-2'-deoxyuridine (5-FdUrd) , (ii) Saamaynta 5-FdUMP fiirsashada ee unugyada intracellular 2'-deoxyuridine-5'-monophosphate (dUMP)/thymidine-5'-monophosphate (TMP) ratio, iyo (iii) heerka dheecaanka 5-FdUMP iyo 5-FU oo ka yimid unugyo dhaqan oo biniaadmi ah oo ay wataan gaadiidleyda ABC.Xaaladeena tijaabada ah, unugyadu waxaa lagu darey 5-FU ama 5-FUrd.Kadibna, borotiinka gacanta waxaa soo dajiyay methanol.Habkani waxa uu bixiyay soo kabasho sare oo soo kabasho ah.Intaa waxaa dheer, si loo cabbiro dheecaanka 5-FU iyo 5-FdUMP ee unugyada, waxaan ku sameynay qiyaasidda molecules-ka dhexdhexaadka ah ee dhaqanka.Warbaahintu si toos ah ayaa loo duray (5-FU) ama waxa la hoos maray weji adag oo soo saarid iyadoo la isticmaalayo Oasis Wax saarista kartoonada (5-FdUMP).Kala-soocidda falanqaynta waxaa lagu sameeyay dC18 Atlantis 3.5 μm, (100 mm × 2.1 mm id) tiir leh qaab isocratic ah iyadoo la adeegsanayo ammonium formate buffer/methanol/water (5/5/90, v/v) sida wejiga wareega.Wakhtiga orodku kama dhaafin 6.2 min.Falanqeeyayaasha waxaa lagu ionized interface electrospray oo hoos yimaada habka ion taban.Waxaan ku ansixinay habka in ka badan 2.5-150 ng mL -1 marka loo eego xeryahooda.Kala duwanaanshiyaha-intra-iyo-kala-duwanaanshaha qiimayntu waxa uu ka hooseeyay 10% todoba maalmood gudahood.Dhammaan xeryahooda waxay ahaayeen kuwo deggan unugyada ama dhex dhexaad dhaqameedka marka shaybaarada lagu kaydiyo -20 °C ugu yaraan laba toddobaad, iyo ka dib saddex wareeg oo baraf-dhalaalid ah.Wax saameyn ah oo matrix ah laguma arag labada warbaahin.