Alaabta

Tet 5-methylcytosine dioxygenases waxay kicisaa demethylation DNA iyagoo soo saaraya 5-hydroxymethylcytosine iyo alaabo kale oo oksaydhaysan.Tet1 iyo Tet2 ayaa si aad ah loogu muujiyay unugyo badan oo awood leh waxaana hoos loo dhigay ilaa xad kala duwan unugyada somatic, laakiin hababka qoraalku ma cadda.Halkan waxaan ku qeexnay xayndaabyada horumariyaha iyo kobcinta ee Tet1 iyo Tet2.Gudaha 15-kb "superenhancer" ee Tet1, waxaa jira laba goobood oo qoraal ah (TSSs) oo leh qaabab firfircooni oo kala duwan inta lagu jiro horumarka.Gobolka kor u kaca ee 6-kb ee TSS-da fog ayaa si aad ah ugu firfircoon unugyada pluripotent-ka ah, si iskeed ah ayuu u soo tebiyaa muujinta Tet1 ee habka transgenic, oo si degdeg ah u mara methylation DNA iyo aamusnaanta marka la kala saaro unugyada dhaqanka iyo epiblast hooyo.TSS labaad oo hoose, oo la xidhiidha dhiirigeliyaha hodanka ku ah CpG ee daciifka ah, waxaa dhaqaajiyay xoojiye deris ah oo ku jira unugyada asliga ah ee uurjiifka ah (ESCs) iyo unugyada epiblast u eg (EpiLCs).Tet2 waxay leedahay romoter-ka jasiiradda CpG oo leh hawlo madax-bannaan oo badan oo badan iyo ESC-u-gaar ah oo kor u qaadaya intragenic fog;kan dambe si degdeg ah ayaa hoos loogu habeeyey EpiLCs.Daraasadayadu waxay daaha ka qaadaysaa habab kala duwan oo nidaam qoraal ah oo ku saabsan Tet1 iyo Tet2 inta lagu jiro kala-guurka gobolka unugga ee horumarka hore.Wariyeyaasha cusub ee is beddelka ah ee isticmaalaya Tet1 iyo Tet2 cis-regulatory domains ayaa laga yaabaa inay u adeegaan si ay u kala soocaan isbeddelada nuanceed ee dawladaha badaniga ah iyo kala duwanaanshaha epigenetic ee hoose.