Alaabta

Caddaynta dhow waxay taageertaa doorka soo ifbaxaya ee β-nicotinamide adenine dinucleotide (β-NAD (+)) sida neurotransmitter cusub iyo neuromodulator ee habdhiska dareenka durugsan -β-NAD (+) ayaa lagu sii daayaa diyaarinta muruqa jilicsan ee dareemayaasha iyo unugyada chromaffin adrenal hab lagu garto neurotransmitter.Hadda ma cadda in tani run u tahay CNS iyo in kale.Isticmaalka qiyaasta qolka yar ee superfusion assay iyo farsamooyinka chromatography dareere-cadaadiska sare leh, waxaan muujineynaa in sare-K (+) kicinta maskaxda hore ee jiirka synaptosomes ay kiciso qulqulka β-NAD (+), adenosine 5'-triphosphate, iyo metabolites adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate, adenosine, ADP-ribose (ADPR) iyo ADPR wareeg ah.Heerka sare-K (+) -ka-soo-baxa qulqulka β-NAD (+) waxaa lagu dhimay kala-goynta SNAP-25 oo leh botulinum neurotoxin A, iyadoo la xakameynayo nooca N-ku-tiirsanaanta Ca (2+) kanaalka ω-conotoxin GVIA , iyo in la joojiyo proton gradient of synaptic vesicles leh bafilo mycin A1, oo soo jeedinaya in β-NAD (+) ay u badan tahay in lagu sii daayo iyada oo loo marayo exocytosis vesicle.Falanqaynta reer galbeedka waxay muujinaysaa in CD38, borotiinka kala duwan ee dheef-shiid kiimikaadka β-NAD (+), uu ku jiro xuubka synaptosomal iyo cytosol.Synaptoosoomyada aan saxda ahayn waxay hoos u dhigaan β-NAD(+) .1,N (6) -etheno-NAD, analoogga fluorescent ee β-NAD (+), waxaa qaata synaptosomes iyo kor u qaadistan waxaa hoos u dhigaya β-NAD (+), laakiin ma aha connexin 43 inhibitor Gap 27. Neurons-ka kortikal codsiyada maxalliga ah ee β-NAD (+) waxay keenaan Ca (2+) ku-meel-gaar ah oo degdeg ah, oo ay u badan tahay inay sabab u tahay qulqulka Ca (2+) ee ka baxsan unugyada.Sidaa darteed, synaptosomes maskaxda jiirka ayaa si firfircoon u sii deyn kara, hoos u dhigi kara oo kor u qaadi kara β-NAD (+), iyo β-NAD (+) waxay kicin karaan neurons-ka postsynaptic, dhammaan shuruudaha looga baahan yahay walaxda si loogu tixgeliyo musharax neurotransmitter maskaxda.