Alaabta

La socodka farmacodynamics marka lagu daro farmasokinetics waa mid ka mid ah xeeladaha lagu shaqsiyeynayo daawaynta mofetil mycophenolate.Ujeedada daraasaddan waxay ahayd in la horumariyo dareeraha xasaasiga ah ee chromatography-tandem mass spectrometry (LC-MS/MS) hababka loogu talagalay qiimaynta farmashiyaha iyo farmashiyaha ee mycophenolic acid (MPA).Isku-duubnaanta mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, iyo sidoo kale MPA iyo MPAG, oo aan la xirin, ayaa la go'aamiyay, iyo inosine-5'-monophosphate dehydrogenase dhaqdhaqaaqa waxaa lagu xisaabiyaa iyadoo la cabbirayo xadiga soo saaray xanthosine-5'-monophosphate (XMP). ) iyo intracellular adenosine-5'-monophosphate ka dib markii la soo saaro unugyada mononuclear-ka ee dhiigga (PBMC) lysates.Tiirka Mastro(TM) ee bilaa birta ah iyo laba qaab oo gradient ah ayaa loo isticmaalay si loo hagaajiyo xadka qiyaasida XMP ilaa 0.1 μM.Kala duwanaanshaha fiirsashada MPA ee bukaan-socodka, toosnaanta qalooca is-xajinta, saxnaanta dhex-iyo-maalin dhexda iyo saxnaanta waxay ku qanacday hab-raacyada Maamulka Cuntada iyo Dawooyinka ee Qudhaanjada.Heerarka MPA ee ku-tallaalidda unugyada unugyada hematopoietic (HSCT) ee bukaannada lagu go'aamiyay baaritaanka enzyme iyo habka LC-MS / MS ee hadda jira ayaa muujiyay xiriir wanaagsan (r (2) = 0.95, p <0.001).Daraasaddan, waxaan ku soo sheegnay hababka xasaasiga ah ee LC-MS/MS si loo qiimeeyo farmashiyaha iyo farmashiyaha MPA, kuwaas oo ku filan xasaasiyad si loo qiimeeyo tiro yar oo PBMC lysates ah oo la ururiyey wax yar ka dib HSCT.