4-Nitrophenyl phosphate disodium cusbo 6-hydrate CAS:333338-18-4 caddaan ilaa budo jaale ah
| Lambarka buugaagta | XD90511 |
| Magaca Alaabta | 4-Nitrofenyl phosphate disodium cusbo 6-hydrate |
| CAS | 333338-18-4 |
| Qaanuunka molecular | C6H16NNa2O12P |
| Miisaanka Molecular | 371.142 |
| Faahfaahinta Kaydinta | Ambient |
| Xeerka Tarifka La Iswaafajiyay | 29199000 |
Tilmaamaha Alaabta
| Muuqashada | cadaan ilaa jaalle cirro leh |
| Qiimayn | 99% |
In ka badan 70% gliomas-hooseeya waxay wataan isku-bedelka heterozygous R132H ee koodka hidda-socodka ee dehydrogenase isocitrate 1 (IDH1).Tani waxay siinaysaa ensaymka awoodda cusub ee loogu beddelayo α-ketoglutarate una beddelo 2-hydroxyglutarate, ugu dambayntiina u horseedda tumorigenesis.Ilaha ugu weyn ee wax soo saarka 2-hydroxyglutarate waa glutamine, kaas oo, kansarka, sidoo kale waa isha wareegga tricarboxylic acid (TCA) anaplerosis.Ilaha kale ee anaplerosis waa qulqulka pyruvate iyada oo loo marayo pyruvate carboxylase (PC), taas oo ah dariiqa caadiga ah ee astrocytes caadiga ah.Hadafka daraasaddan ayaa ahayd in la go'aamiyo in PC-gu u adeego ilaha TCA anaplerosis ee unugyada mutant ee IDH1 halkaas oo glutamine loo isticmaalo wax soo saarka 2-hydroxyglutarate.Qulqulka pyruvate ee loo sii marayo PC iyo pyruvate dehydrogenase (PDH) waxaa lagu go'aamiyay iyadoo la isticmaalayo spectroscopy resonance magnetic si loo baadho sumaynta [2-¹³C]glucose-ka ka soo jeeda ¹³C-labe led glutamate iyo glutamine.Qiimaynta dhaqdhaqaaqa, RT-PCR iyo xuubka reer galbeedka ayaa loo isticmaalay in lagu baadho muujinta iyo waxqabadka enzymes khuseeya.Xogta Kansarka Genome Atlas (TCGA) ayaa la falanqeeyay si loo qiimeeyo muujinta enzymes ee muunadaha glioma ee bini'aadamka.Marka la barbar dhigo unugyada nooca duurjoogta ah, unugyada IDH1 ee mutant waxay si weyn u kordhiyeen qulqulka jajabka ee PC.Tani waxay la xidhiidhay korodhka weyn ee dhaqdhaqaaqa PC iyo muujinta.Isla markaa, dhaqdhaqaaqa PDH si weyn ayuu hoos ugu dhacay, oo ay u badan tahay in ay dhexdhexaadinayaan inhibitory PDH fosphorylation aad u kordhay by PDH kinase 3. Iyadoo la raacayo fiirsashada unugyada, falanqaynta xogta TCGA ayaa muujisay koror weyn oo ku saabsan muujinta PC ee mutant IDH-muujinta muunadaha glioma bini'aadamka marka loo eego duurjoogta- nooca IDH.Natiijooyinkayagu waxay soo jeedinayaan in isbeddelada PC iyo PDH ay noqon karaan qayb muhiim ah oo ka mid ah la qabsiga gacanta ee isbeddelka IDH1 waxayna u adeegi karaan bartilmaameedyo daweyn oo suurtagal ah.
