Alaabta

Xeryahooda Multitarget ayaa si sii kordheysa loogu raadinayo daaweynta wax ku oolka ah ee cudurrada kakan.Halkan, waxaan ku qeexeynaa naqshadeynta iyo isku dhafka fasalka cusub ee shogaol-huprine hybrids, oo la sheegay in lagu dhuftay dhowr bartilmaameed oo muhiim ah oo ku lug leh cudurka Alzheimers.Isku-dhafkan ayaa lagu tijaabiyay in vitro si ay u qabtaan waxqabadkooda ka hortagga acetylcholinesterase bini'aadamka iyo butyrylcholinesterase iyo waxqabadka antioxidant (ABTS.+, DPPH iyo Folin-Ciocalteu assays), iyo unugyada Escherichia coli ee aan qummanayn ee Aβ42 iyo tau waxqabadyada isku-ururinta.Sidoo kale, dhexgalka maskaxdooda ayaa la qiimeeyay (PAMPA-BBB assay).In kasta oo isku-dhafkan uusan ahayn kuwa awood u leh AChE inhibitors ama wakiilada antioxidant sida waalidka huprine Y iyo [4] -shogaol, siday u kala horreeyaan, waxay weli muujinayaan firfircoonida anticholinesterase iyo antioxidant aad u xoog badan waxayna aad uga awood badan yihiin Aβ42 iyo tau wakiilada isku-ururinta marka loo eego xeryahooda waalidka.Guud ahaan, isku-dhafka shogaol-huprine waxay u soo baxaan sidii maskax xiiso leh oo laga gudbi karo bartilmaameedyada badan ee ka hortagga Alzheimers.

Soo saarida polarity kala duwan oo laga helay qaybaha dhirta kala duwan (xidid, caleen, ubaxa iyo miro) ee Seseli rigidum waxaa lagu bartay by antioksidanter kala duwan: DPPH iyo ABTS dhaqdhaqaaqa scavenging xagjirka ah, by wadarta hoos u dhigista habka awoodda iyo sidoo kale iyada oo loo marayo wadarta guud ee flavonoids iyo polyphenols. .Saliidaha muhiimka ah ee dhammaan qaybaha dhirta waxay muujiyeen sifooyinka antioxidant daciif ah.Kala duwanaanshaha xakamaynta xakamaynta ee soosaarayaasha la tijaabiyay, ee ka soo horjeeda bakteeriyada Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, iyo fangaska Candida albicans iyo Aspergillus niger waxay ahayd 0.01-1.50 mg / mL iyo microbicidal 0.02-3.00 mg / ml.In dhexgalka cholinesterase, dhammaan saliidaha lagama maarmaanka ah ayaa caddeeyey waxtar leh sida ka hortagga.Boqolkiiba ugu sarreeya ee xannibaadda ka soo horjeeda cholinesterase aadanaha iyo faraska waxaa lagu muujiyay saliidda asaasiga ah (38.20% iyo 48.30%, siday u kala horreeyaan) ee saliidda, iyo xididka hexane (40.56% iyo 50.65% siday u kala horreeyaan).Saliidaha muhiimka ah iyo qaybaha isbedbeddelaya ee dhammaan qaybaha dhirta waxaa aqoonsaday GC, GC-MS iyo madaxa/GC-MS.Falanqaynta tirakoobka ee isu-ururinta natiijooyinka ayaa muujisay in halabuurka saliidda aasaasiga ah ee xididku uu si weyn uga duwan yahay saliidaha lagama maarmaanka ah ee qaybaha kale ee geedka.Iyadoo la tixgelinayo dhammaan waxqabadyada la bartay, xididka hexane ee xididku wuxuu muujiyay sifooyinka guud ee ugu fiican.Iyada oo la adeegsanayo chromatography dareeraha waxqabadka sare leh oo ay weheliso spectrometry mass spectrometry xallinta sare, 30 ka qaybood ee ugu badan ayaa lagu aqoonsaday soosaaryo kala duwanaansho ah.Joogitaanka walxaha la aqoonsaday ayaa lala xiriiriyay dhaqdhaqaaqyo bayooloji oo gaar ah oo la arkay, sidaas darteed la qoondeeyay xeryahooda mas'uul ka ah waxqabad kasta oo la soo bandhigo.