Alaabta

LecA (PA-IL) waa lectin cytotoxic ah iyo adhesin ay soo saartay Pseudomonas aeruginosa kaas oo ku xidha galactosides hydrophobic oo leh gaar gaar ah iyo xiriir sare.Adigoo isticmaalaya isku-dhafka tarjumaadda lecA-egfp iyo falanqaynta immunoblot ee matrixka biofilm ee ka baxsan unugyada, waxaan tuseynaa in lecA lagu muujiyo unugyada biofilm-koray.Qiimaynta biofilm static ee labada polystyrene iyo birta, qotodheerta biofilm iyo daboolida dusha sare waxaa hoos u dhigay isbeddelka lecA waxaana lagu xoojiyey LecA-soo-saarka xad dhaafka ah PAO-P47.Daboolida dusha sare ee Biofilm ee cadaadiska waalidka, PAO-P47 laakiin maaha mutant lecA ee kuboonnada birta sidoo kale waxaa xannibay koritaanka joogitaanka isopropyl-beta-D-thiogalactoside (IPTG) ama p-nitrophenyl-alpha-D-galactoside ( NPG).Intaa waxaa dheer, bayooloji-nooca duurjoogta ah ee qaan-gaaray ee la sameeyay maqnaanshaha galactosides-ka hydrophobic waxaa lagu kala firdhi karaa marka lagu daro IPTG.Taas bedelkeeda, ku-darka p-nitrophenyl-alpha-L-fucose (NPF) oo leh xiriir sare oo loogu talagalay P. aeruginosa LecB (PA-IIL) lecti n wax saameyn ah kuma yeelan samaynta biofilm ama kala firdhinta.Kobaca Planktonic ee P. aeruginosa PAO1 wax saameyn ah kuma yeelan joogitaanka IPTG, NPG ama NPF, mana ahayn culeyska awood u leh inuu isticmaalo sonkortaas ilaha kaarboonka, taasoo soo jeedinaysa in saameynta la arkay ee sameynta biofilm ay sabab u tahay joojinta tartanka ee LecA-ligand xirid.Natiijooyin la mid ah ayaa sidoo kale laga helay biofilms oo ku koray xaaladaha qulqulka firfircoon ee rasiidhada birta, taas oo soo jeedinaysa in LecA ay gacan ka geysato dhismaha P. aeruginosa biofilm ee xaaladaha deegaanka ee kala duwan.

Alfa-L-fucosidase (EC 3.2.1.51) oo awood u leh inuu sii daayo haraaga t-fucosyl ee silsiladda dhinaca ee xyloglucan oligosaccharides ayaa laga helay caleemaha dhirta Arabidopsis.Waxaa intaa dheer, alfa-L-fucosidase oo leh sifooyin substrate la mid ah ayaa laga sifeeyay kaabajka (Brassica oleracea) caleemaha si loogu sameeyo hal koox oo SDS-PAGE ah.Laba qaybood oo peptide ah ayaa laga helay kooxdan borotiinka ah, waxaana loo isticmaalay in lagu aqoonsado hiddo-wadaha Arabidopsis ee alfa-fucosidase oo aanu u soo jeedinay in lagu waco AtFXG1.Intaa waxaa dheer, hiddo-wadaha Carabidopsis oo leh homology-ga loo yaqaan alfa-L-fucosidases ayaa sidoo kale la helay, waxaanan soo jeedinay inaan u magacowno sida AtFUC1.Labada AtFXG1 iyo ATFUC1 ayaa si kala duwan loogu muujiyay unugyada wadaaddada Pichia iyo hawlaha alfa-L-fucosidase ee qarsoodiga ah ee dhexdhexaadinta dhaqanka.Alfa-L-fucosidase-ku-qoray AtFXG1 wuxuu ahaa mid firfircoon oo ka soo horjeeda oligosaccharides ee xyloglucan XXFG iyo sidoo kale ka soo horjeeda 2'-fucosyl-lactitol laakiin kama soo horjeedo p-nitrophenyl-alpha-L-fucopyranoside.Si kastaba ha ahaatee, AtFUC1 heterologically ayaa lagu muujiyay firfircooni kaliya ee ka soo horjeeda 2'-fucosyl-lactitol.Sidaa darteed, kii hore waa inuu la xiriiraa dheef-shiid kiimikaadka xyloglucan.