Alaabta

Daraasadda hadda jirta waxay qeexaysaa hab fudud oo hufan oo loo adeegsado chromatography dareeraha waxqabadka sare (HPLC) oo ay weheliso ogaanshaha fluorescence ee go'aaminta cabbirrada kinetic ee qaadashada glutamate ee unugyada dareemayaasha.Unugyada maqaarka ee laga helay chicks 7-maalmood jir ah ayaa lagu dhex daray glutamate-ka la yaqaan (50-2000 μM) ee 10 min, iyo heerarka o-phtaldehyde (OPA) -derivatized neurotransmitter ee dhexdhexaadinta ka-soo-baxa ayaa la qiyaasay.Iyadoo la qiimeynayo farqiga u dhexeeya uruurinta bilowga iyo kama-dambaysta ah ee glutamate ee dhexdhexaadka ah, habka kor-u-qaadista la qaadan karo ayaa lagu gartaa (K (m) = 8.2 iyo V (max) = 9.8 nmol / mg protein / min).Cabbirkani waxa uu ahaa mid inta badan soodhiyam- iyo heerkul-ku-tiirsane, si xoog leh u taageeraya in habka hoos u fiirsiga ay dhab ahaantii qaataan gaadiidleyda xidhiidhka-sare leh.Marka lagu daro tan, natiijooyinkayagu waxay sidoo kale muujiyeen in zinc chloride (horjoogaha glutamate / gaadiidleyda aspartate) ay kicisay hoos u dhac ku-tiirsanaanta ku-tiirsanaanta qaadashada glutama te, taasoo muujineysa sida gaarka ah ee habkayaga.Guud ahaan, shaqada hadda jirta waxay tilmaamaysaa hab kale oo lagu qiimeeyo qaadashada glutamate ee unugyada neerfayaasha iyadoo la adeegsanayo HPLC.Habkani wuxuu noqon karaa qalab muhiim ah oo loogu talagalay daraasadaha la xidhiidha dabeecadda isbeddelka daqiiqadaha ee gaadiidka glutamate ee la xidhiidha dhaawaca habka dhexe ee neerfaha.