Proteinase K albamka tritirachium Cas: 39450-01-6 99% budo cad
Lambarka buugaagta | XD90434 |
Magaca Alaabta | Proteinase K ee albamka tritirachium |
CAS | 39450-01-6 |
Qaanuunka molecular | C29H27N2O12P |
Miisaanka Molecular | 626.50468 |
Faahfaahinta Kaydinta | -15 ilaa -20 °C |
Xeerka Tarifka La Iswaafajiyay | 35079090 |
Tilmaamaha Alaabta
Cufis-jiidad gaar ah | 40un/mg borotiin min |
Dhaqdhaqaaqa Enzyme | 30un/mg min miisaan qallalan |
Muuqashada | budo cad |
Qiimayn | ≥99% |
Cilmi-baadhisyada difaaca jirka ee chromatin-wide-wide (ChiP) waxay keeneen aragti weyn oo ku saabsan deegaan-genomic ee borotiinnada la xidhiidha chromatin iyo isbeddellada histone.Si kastaba ha ahaatee, tirada badan ee xogta ay keeneen falanqayntaasi, si kastaba ha ahaatee, waxay u baahan yihiin habab suurtagelinaya in si degdeg ah loo ansixiyo iyo falanqaynta ku habboonaanta noolaha.Intaa waxaa dheer, waxaa weli jira bartilmaameedyada borotiinka iyo beddelka kuwaas oo ay adag tahay in la ogaado iyadoo la adeegsanayo hababka ChIP ee caadiga ah.Si wax looga qabto arrimahan, waxaan sameynay nidaam is-difaacid degdeg ah oo chromatin ah kaas oo aan u yeerno ZipChip.ZipChip waxay si weyn u yaraynaysaa wakhtiga waxayna kordhisaa dareenka u oggolaanaya in si degdeg ah loo baaro meelo badan.Halkan waxaan ku qeexaynaa sida ZipChIP ay awood u siiso in la ogaado wax ka beddelka histone (H3K4 mono- iyo trimethylation) iyo laba khamiir histone demethylases, Jhd2 iyo Rph1, kuwaas oo markii hore ay adkeyd in la ogaado iyadoo la isticmaalayo hababka caadiga ah.Intaa waxaa dheer, waxaan muujineynaa kala duwanaanshaha ZipChIP anagoo falanqeyneyno kobcinta histone deacet ylase Sir2 ee heterochromatin ee khamiirka iyo kobcinta dib u habeynta chromatin, PICKLE, ee euchromatin ee Arabidopsis thaliana.