Proteinase K Cas: 39450-01-6 budo cad
Lambarka buugaagta | XD90191 |
Magaca Alaabta | Proteinase K |
CAS | 39450-01-6 |
Qaanuunka molecular | C29H27N2O12P |
Miisaanka Molecular | 626.50 |
Faahfaahinta Kaydinta | -15 ilaa -20 °C |
Xeerka Tarifka La Iswaafajiyay | 35079090 |
Tilmaamaha Alaabta
Lambarka buugaagta | XD90191 |
Magaca Alaabta | Proteinase K |
CAS | 39450-01-6 |
Qaanuunka molecular | C29H27N2O12P |
Miisaanka Molecular | 626.50 |
Faahfaahinta Kaydinta | -15 ilaa -20 °C |
Xeerka Tarifka La Iswaafajiyay | 35079090 |
Inta badan xayawaanka leh X iyo Y koromosoomyada jinsiga, hababka koromosoomyada ballaaran ayaa loo adeegsadaa si loo dheellitiro muujinta hidde-sidaha X-ku xiran ee ragga iyo dumarka.Duqsiga Drosophila melanogaster, habka magdhowga qiyaasta ayaa sidoo kale guud ahaan ku fidiya transgenes-ku xiran ee X.In ka badan 70 xariiq oo transgenic ah oo ka mid ah idaha Australiyaanka ah ee afuufaya Lucilia cuprina ayaa la sameeyay iyada oo qayb ka ah dadaalka lagu horumarinayo noocyada lab-kaliya ee barnaamijka xakamaynta hidda-socodka ee cayayaankan weyn ee idaha.Dhammaan khadadka waxay wataan hiddo-wadaha calaamadaynta borotiinka oo si habaysan loo muujiyey.Dhammaan 12-ka xariiq ee X-kuxiran, dirxiga dheddigga ah ayaa muujinaya fluorescence ka sii ifaya marka loo eego dirxiga labka ah, taasoo soo jeedinaysa in hidde-sideyaasha aan la magdhabin.Tan waxaa lagu xaqiijiyay tirada RT-PCR ee khadadka la doortay.Si loo go'aamiyo haddii hiddo-wadaha X-kuxiran ee endogenous la magdhabay, waxaanu ka soocnay 8 hiddo-sideyaal kuwaas oo ah orthologs ee hiddo-wadaha kuwaas oo ku jira koromosoomka afraad ee D. melanogaster.Caddaynta dhawaa waxay soo jeedinaysaa in koromosoomka afraad ee D. melanogaster, ama element Muller F, uu yahay koromosoomka awoowaha X ee ku jira Diptera kaas oo dib ugu noqday autosome ee noocyada Drosophila.Waxaan ku tusineynaa tirada PCR ee DNA-da lab iyo dhedig in 6 ka mid ah 8da kooxda isku xira F hiddo-wadaha ay ku nool yihiin X koromosoomyada L. cuprina.Labada kale ee Muller element F hiddo-wadaha waxaa laga helay inay yihiin autosomal gudaha L. cuprina, halka laba hidde-sideyaasha Muller element B laga helay isla gobolka X koromosoomka sida L. cuprina orthologs ee D. melanogaster Ephrin iyo gawky genes.Waxaan ogaanay in hiddo-wadaha L. cuprina X koromosoomyada ay si isku mid ah u muujiyaan ragga iyo dheddigga (ie, qiyaasta qiyaasta la magdhabay).Sidaa darteed, si ka duwan Drosophila, waxay u muuqataa in nidaamka magdhowga qiyaasta Lucilia uu gaar u yahay hiddo-wadaha ku xiran koromosoomka X oo aan la isku xiri karin transgenes dhawaan yimid.