Alaabta

Xubinta dheecaanka borotiinka ee ExPortal ee Streptococcus pyogenes waa xuubka phospholipid-ka anonic-phospholipid ka kooban microdomain ka kooban Sec translocons iyo arrimo biogenesis borotiinka ka dib.Polymyxin B waxay ku xidhaa oo carqaladaysaa daacadnimada ExPortal, taasoo keentay dheecaanka cilladaysan ee dhowr sun ah.Si loo helo fahanka arrimaha saameeya ururka ExPortal, shaashad hidde-side ah ayaa la sameeyay si loo doorto mutantiyada iska caabbinta polymyxin B ee muujinaya daacadnimada ExPortal oo la xoojiyey.Guud ahaan-genome-ga isku xigxiga ee 25 mutants adkaysi u leh ayaa shaaca laga qaaday min hal ilaa afar beddelka hidde-sideyaasha noole oo inta badan ku urursan xudunta u ah 10 kooxood oo hidde-sideyaal ah.Dhisidda mutants ee leh tirtirid gaar ah ama gelinta gelinta ayaa muujisay in 7 hiddo-sideyaasha xudunta u ah ay keenaan iska caabin iyo wanaajinta daacadnimada ExPortal iyada oo loo marayo luminta shaqada, halka 3 ay u badan tahay inay sabab u tahay helitaanka shaqada iyo/ama saameynaha isku dhafan.Hidde-sideyaasha iska caabbinta xudunta u ah waxaa ka mid ah nidaamiyaha qoraalka ee biosynthesis ee lipid, hiddo-sideyaal dhowr ah oo ku lug leh helitaanka nafaqeynta, iyo noocyo kala duwan oo hidde-sideyaal ah oo ku lug leh jawaabaha walaaca.Laba xubnood oo ka tirsan fasalka dambe ayaa sidoo kale u shaqeeya sidii nidaamiyayaal cusub oo ah borotiinka SpeB cysteine-ka ee qarsoon.Falanqaynta isku-beddelka go'doonsan ee inta badan, hal nukleotide tirtirka ee raad ah 9 isku xigta hadhaaga adenine ee pstS, codaynta qayb ka mid ah gaadiidka Pi-gaaban, waxay soo jeedinaysaa in taxanahani uu u shaqeeyo sidii beddelka molecular si loo fududeeyo la qabsiga walaaca.Si wada jir ah, xogtani waxay soo jeedinaysaa jiritaanka jawaab celin diiqad xuub ah oo kor u qaadaysa daacadnimada ExPortal iyo iska caabinta cationic antimicrobial peptides.