Alaabta

PIPES [piperazine-N, N'-bis(2-ethanesulfonic acid)] ayaa inta badan loo isticmaalaa sidii wakiilka kaydiya ee biochemistry.Waa kaydka ethanesulfonic acid ee ay soo saartay Good et al.1960-kii.PIPES waxay leedahay pKa u dhow pH physiological taasoo ka dhigaysa mid waxtar u leh shaqada dhaqanka unugyada.Waxaa la diiwaangeliyay si loo yareeyo luminta dufanka marka la buufiyo glutaraldehyde histology ee unugyada dhirta iyo xayawaanka.Wakiil kaydiya oo leh pKa u dhow pH jireed.

Antimicrobial peptide magainin 2 waxay samaysaa daloolo xuubka dufanka leh waxayna keentaa dalool xuubka ku jira unugyada gacanta.In kasta oo daloolladani ay u badan tahay sababta ugu weyn ee hawlaheeda bakteeriyada, habka samayska daloolka ayaa weli ah mid si liidata loo fahmay.Sidaa awgeed waxaanu si faahfaahsan u baadhnay is dhexgalka magainin 2 iyo xuubabka dufanka leh anagoo adeegsanayna hal-abuuro unilamellar vesicles (GUVs).Isku-xidhka magainin 2 ee xuubka dufanka ee GUVs wuxuu kordhiyey isbeddelka jajabka ee aagga xuubka, δ, taas oo u dhiganta fiirsashada dusha sare ee magain 2, X. Tani waxay muujinaysaa in heerka joogtada ah ee magain 2-laba keenay -is-beddelka gobolka ee ka soo baxa xaaladda dhabta ah ee gobolka pore ayaa si weyn u kordhay kororka δ.Xiisadda xuubka dufanka ee ka dib hamiga GUV ayaa sidoo kale hawl galiyay samayska daloolka 2-magainin.Si loo muujiyo meesha uu joogo magainin 2, is dhexgalka carboxyfluorescein (CF) -ku calaamadsan magainin 2 (CF-magain 2) oo leh hal GUVs oo ay ku jiraan baaritaanka fluorescent biyo-milmi leh, AF647, ayaa la baaray iyadoo la adeegsanayo microscopy confocal.Maqnaanshaha xiisadda sababtoo ah rabitaanka, ka dib isdhexgalka magainin 2 xoojinta fluorescence ee rim GUV sababtoo ah CF-magainin 2 ayaa si degdeg ah u kordhay qiimaha joogtada ah, kaas oo sii jiray muddo dheer, iyo 4-32 s ka hor. Bilawga daadinta AF647 xooga geesku wuxuu bilaabay inuu si degdeg ah u kordho qiimo kale oo joogto ah.Taas bedelkeeda, joogitaanka xiisadda, wax koror ah oo ku saabsan xoojinta cirifka wax yar ka hor inta aan la arkin bilawga qulqulka.Natiijooyinkani waxay muujinayaan in magainin 2 uusan ka beddeli karin dibadda una guuri kara monolayer gudaha ilaa wax yar ka hor abuuritaanka daloolka.Iyada oo ku saleysan natiijooyinkaan, waxaan ku soo gabagabeyneynaa in 2-daloolada magainin ay tahay dalool fidsan oo firfircoon iyo fidinta monolayer-ka gudaha ayaa ah xoogga ugu muhiimsan ee abuuritaanka daloolka.