Alaabta

Inkasta oo fluoride la og yahay inuu kiciyo samaynta lafaha, hababka hoose si buuxda looma fahmin.Daraasadihii ugu dambeeyay waxay saameyn ku yeesheen dariiqa Wnt/β-catenin inay tahay calaamad weyn oo calaamad u ah bayoolaji lafaha.Daraasadadeenii hore waxay iftiimiyeen doorka suurtagalka ah ee dariiqa Wnt ee samaynta lafaha ee jiirka soo ifbaxa fluoride, laakiin habka ayaa weli ah mid aan caddayn.Daraasadda hadda jirta ayaa go'aamisay ka-qaybgalka calaamadaha Wnt/β-catenin ee kala-duwanaanta osteoblastic-ka ee fluoride-ku keeno.Isticmaalka osteoblasts-ka jiirka aasaasiga ah, waxaanu muujinay in fluoride uu si weyn u dhiirrigeliyay bararka osteoblasts iyo muujinta phosphate alkaline (ALP) iyo sidoo kale heerarka muujinta mRNA ee calaamadaha kala soocida lafaha, oo ay ku jiraan nooca I kolajka (COL1A1), ALP iyo osteonectin.Waxaan sidoo kale ka helnay fosforyaalka fluoride-ka ee serine 473 ee Akt iyo serine 9 ee glycogen synthase kinase-3β (GSK3β), taas oo keentay in GSK-3β la joojiyo iyo ka dib ururinta nukliyeerka ee β-catenin, sida lagu muujiyay Western blot iyo falanqaynta immunofluorescence. .Intaa waxaa dheer, fluoride sidoo kale waxay keentay muujinta Wnt-bartilmaameedka hidda-wadaha runt ee la xidhiidha qoraalka 2 (Runx2).Muhiimad ahaan, saamaynta togan ee fluoride ee waxqabadka ALP iyo tibaaxaha mRNA ee COL1A1, ALP, osteonection iyo Runx2 waxaa baabi'iyay DKK-1, xannibaadaha Wnt/β-catenin reseptor.Isku soo wada duuboo, natiijooyinkani waxay soo jeedinayaan in fluoride ay kor u qaaddo kala soocida osteoblastic iyada oo loo marayo Akt-iyo GSK-3β-ku-tiirsanaanta dariiqa calaamadaha Wnt/β-catenin ee osteoblasts-ka aasaasiga ah.Natiijooyinkayagu waxay bixiyaan fikrado cusub oo ku saabsan hababka ficilka fluoride ee osteoblastogenesis.