Alaabta

Nidaam adag oo difaaca jirka ah oo loogu talagalay go'aaminta progesterone ee balasmaha bini'aadamka ayaa lagu sifeeyay, kaas oo u isticmaala chemiluminescence sida barta dhamaadka.Isku-xidhaha progesterone-isoluminol wuxuu u adeegaa sidii calaamadeeyaha kiimikaalka.Qayb IgG ee antiserum ilaa progesterone-11 alfa-hemisuccinate bovine serum albumin ayaa si aan toos ahayn loogu dhajiyay gidaarada Lumacuvettes P tuubooyinka tijaabada polystyrene.Ka dib falcelinta isku xidhka ah, cuvettes waa la dhaqaa.Wax-soosaarka iftiinka ee isku-xidhka isku-xidhka oksaydhka ee leh nidaamka H2O2-microperoxidase ee pH 13 ayaa hoos u dhacay iyadoo la kordhinayo xoojinta progesterone-ka bilaashka ah ee hab-loog-linear ah inta u dhaxaysa 15-1000 pg/tube.Chemiluminescence immunoassay waxay la mid tahay radioimmunoassay marka loo eego dareenka, gaarnimada, saxnaanta iyo saxnaanta.Imtixaanku wuxuu bixiyaa faa'iidooyinka xawaaraha iyo sahlanaanta otomaatiga.Intaa waxa dheer, habkani kuma lug laha isticmaalka radioisotopes ama tallaabo centrifugation.