Alaabta

Habka kinetic angiotensin-converting enzyme (ACE), bakhaar la taaban karo oo wanaagsan waa 80 mmol/L borate baffer pH 8.2 (37 darajo C).Marxaladda daahitaanka ayaa lagu ogaadaa falcelinta, iyo 5-daqiiqo ku-soo-baxa substrate iyo balaasmaha ayaa la soo jeediyay ka hor cabbirka kinetic-ka.Substrate-ka, N-[3- (2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), xooga saarista waxaa lagu kordhiyey 1.0 mmol/L iyo hirarka dhererka dhererka waa 345 nm si loo xaqiijiyo toosnaanta cabbirka.Habka la soo jeediyay wuxuu isticmaalayaa 1:9 balaasmaha-ilaa-reagent saamiga.Kala duwanaanshaha tooska ah ee baadhistu waxa ay ku fidsan tahay ku dhawaad ​​170 U/L, taas oo ka dhigan 25% hydrolysis substrate ah.Nuugista FAPGG waxaa lagu go'aamiyaa iyadoo la cabbirayo farqiga u dhexeeya nuugista 1.0 mmol/L FAPGG iyo xalalka alaabta.Aaminimada hirarka dhererka waxaa lagu hubiyaa iyadoo la ogaanayo qiimaha nuugista ee la filayo ee xalka FAPGG, iyo leexashada 1.0-nm ee 345 nm waxay beddeshaa nuugista 15.5%.Saxnaanta ACE ee qiyaasaha 60 iyo 100 U/L waa 3.5% iyo 2.4% dufcad gudaheed iyo 2.9% iyo 2.6% inta u dhaxaysa dufcada, siday u kala horreeyaan.Inta u dhaxaysa tixraaca (2.5th ilaa 97.5th percentiles) waa 41-139 U/L, mana jiro farqi u dhexeeya qiyamka ragga iyo dumarka.