Alaabta

Kaarbonic anhydrase activators: daraasadii ugu horeysay ee firfircoonida ee isoformy secretory aadanaha VI oo leh asiidhyada amino iyo amines.

isozyme sirta ah ee anhydrase kaarboonka bini'aadamka (hCA, EC 4.2.1.1), hCA VI, ayaa la isku duubay, la muujiyay, oo lagu nadiifiyay nidaamka muujinta bakteeriyada.Qiyaasaha kinetic ee CO(2) falcelinta fuuq-celinta waxay caddeeyeen hCA VI inay lahaato ak (bisad) 3.4 x 10(5) s (-1) iyo k (bisad)/K (M) ee 4.9 x 10(7)M (-1) s (-1) (oo ah pH 7.5 iyo 20 degrees C).hCA VI waxay leedahay firfircooni firfircoon oo muhiim ah oo loogu talagalay falcelinta jireed, ee isku xiga ee baaxadda sida isoform CA I ama transmembrane, isozyme CA IX ee buro la xidhiidha.Taxanayaal amino acids iyo amines ah ayaa la tusay inay u dhaqmaan sidii CA VI activators, oo leh waxqabadyo kala duwan.l-His, l-Trp, iyo dopamine waxay muujiyeen daciifnimada CA VI firfircoonida (K(A) s ee 21-42 microM), halka d-His, d-Phe, l-DOPA, l-Trp, serotonin , iyo qaar ka mid ah pyridyl-alkylamines ayaa ahaa firfircooniyaal ka wanaagsan, iyadoo K(A) ay ku dhex jiraan 13-19 microM.Dhaqdhaqaaqayaasha ugu fiican ee CA VI waxay ahaayeen l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, iyo histamine, oo leh K(A) inta u dhaxaysa 1.23-9.31 microM.Dhammaan hawl-wadeennadani waxay kor u qaadaan k (bisad), iyagoo aan wax saameyn ah ku yeelan K (M), ka qaybqaadashada sidaas awgeed heerka go'aaminta tallaabada wareegga wareegga, falcelinta proton-ka ee u dhexeeya goobta firfircoon ee enzyme iyo deegaanka.