Alaabta

Biotransformation of methyl-β-D-glucopyranoside ilaa silsilad sare alkyl glucosides by unug ku xidhan β-glucosidase of Pichia echellsii.

Daraasadda hadda jirta, waxaan baarnay isticmaalka Pichia echellsii unugyada oo dhan ee loogu talagalay isku-darka silsiladaha alkyl glucosides.Methyl-β-d-glucopyranoside (MG) ayaa loo isticmaalay falcelinta alkohols dufan leh, n-hexanol, n-octanol, n-decanol iyo n-dodecanol si loo soo saaro alkyl glucosides.Xaaladaha falcelinta bilawga ah ayaa marka hore lagu hagaajiyay qiyaasta 2.5 ml ee isku dhafka octyl glucoside (OG) waxayna ahaayeen 8% biyaha, 100mM MG iyo 6h ee wakhtiga falcelinta tanina waxay keentay ≈ 53% dhalidda.Qiyaasta transglucosylation/hydrolysis ugu badnaan ee 2.79 ayaa laga helay 100mM MG oo door biday wax soo saarka sare.Iyada oo ku saleysan shuruudaha la hagaajiyay, reactor ayaa lagu qalay heer 50 ml taas oo keentay ≈ 60% beddelka MG ee OG.Habka chromatography dareeraha fudud ee waxqabadka sare ayaa loo sameeyay si loo qiyaaso glukosides silsiladda sare iyadoo la adeegsanayo qalabka wax sheegta.Ugu badnaan 27% iyo 13% ayaa la helay decyl-, iyo dodecyl-β-d-glucopyranoside, siday u kala horreeyaan.