Alaabta

Si la mid ah fayrasyada RNA-ga ee togan, tombusvirus-yada waxaa lagu soo celiyaa xuubka-ku-xidhan kaabista fayraska (VRC).VRC-gu waxa uu ka kooban yahay p92 fayraska- summadaysan RNA-ku-tiirsanaanta RNA polymerase (RdRp), fayraska p33 RNA chaperone, iyo borotiinada martida loo yahay ee la doortay.Si loo noqdo RdRp shaqaynaysa tarjumaadda ka dib, borotiinka soo-celinta p92 waa in lagu daraa VRC, oo ay ku xigto hawlgelintiisa.Waxaan horey ugu muujinnay qiimeyn ku saleysan soosaar khamiir-la'aan ah oo unug ah in kicinta fayraska stunt ee yaanyada bushy (TBSV) RdRp ay u baahan tahay cunsur martigelinaya oo milmi kara.Maqaalkan, waxaan ku aqoonsaneynaa borotiinka shoogga kulaylka gacanta ee 70 (Hsp70) inuu yahay cunsur martigeliyaha la doortay ee looga baahan yahay kicinta TBSV RdRp N-dambe go'ay.Intaa waxaa dheer, xannibaadda-molecule-ku-salaysan ee shaqada Hsp70 waxay joojisaa isku-darka RNA ee tombusvirus RdRp in vitro.Intaa waxaa dheer, waxaan ku tusineynaa fosfoolipids dhexdhexaad ah, kuwaas oo ah, phosphatidylethanolamine (PE) iyo phosphatidylcholine (PC), kor u qaadista RdRp ee vitro.Taas bedelkeeda, phosphatidylglycerol (PG) waxay muujinaysaa saameyn xooggan oo ka hortag ah oo ku saabsan firfircoonida in vitro RdRp.Waxaan sidoo kale muujineynaa in PE iyo PC ay kiciyaan isdhexgalka RdRp-viral iyo strand RNA [(+) RNA], halka PG ay joojiso ku-xidhka fayraska RNA ee RdRp.Iyada oo ku saleysan doorka kicinta iyo ka-hortagga ee fosfolipids kala duwan ee firfircoonida tombusvirus RdRp, waxaan soo jeedineynaa in ka kooban dufanka xuub-hoosaadka ee bartilmaameedka ah laga yaabo in ay isticmaalaan tombusviruses si loo xakameeyo kulanka cusub ee VRC inta lagu jiro muddada caabuqa. (RdRp), kaas oo mas'uul ka ah isku-darka faraca RNA ee fayraska ee unugyada cudurka qaba ee dhowr fayraska RNA-ga togan, ayaa markii hore aan shaqayn.Istaraatiijiyadani waxay u badan tahay inay ka fogaato isku-darka fayraska RNA ee cytosol kaas oo si degdeg ah u horseedi doona kicinta jawaabaha ka hortagga fayraska gacanta ee RNA.Inta lagu guda jiro isku dhafka isku duubnida xuub-ku-xidhan, fayraska RdRp wuxuu noqdaa mid hawlgeliya iyada oo loo marayo hannaan aan dhammaystirnayn oo la fahmi karo oo ka dhigaya RdRp mid awood u leh isku-darka RNA.Isticmaalka TBSV RdRp, waxaan tuseynaa in Hsp70 chaperone-ka gacanta ee la isku daray iyo fosfoolipids dhexdhexaad ah ay fududeeyaan firfircoonida RdRp gudaha vitro.Taas bedelkeeda, phosphatidylglycerol (PG) waxay leedahay saameyn hor leh oo ka hortag ah oo ku saabsan firfircoonida in vitro RdRp iyo isdhexgalka RdRp-viral RNA, taas oo soo jeedinaysa in microdomain-ka membranous ee ku hareeraysan RdRp uu si weyn u saameeyay kartidiisa isku-dhafka RNA.Markaa, firfircoonida RdRp fayraska waxay u badan tahay inay ku xidhan tahay qaybo badan oo martida loo yahay ee unugyada cudurka qaba.