Alaabta

Waxa aanu samaynay hannaan wax-is-gaarsiineed oo yar oo faragelinaya RNA (siRNA) annagoo adeegsanayna dhisme ternary ah oo leh polyethyleneimine (PEI) iyo γ-polyglutamic acid (γ-PGA), kaasoo muujiyay saamaynta aamusnaanta oo aan lahayn cytotoxicity.Dhismayaasha binary ee siRNA ee leh PEI waxay ahaayeen ku dhawaad ​​73-102 nm cabbirka walxaha iyo 45-52 mV oo ζ- macquul ah.Saamaynta aamusnaanta ee dhismayaasha siRNA/PEI waxay korodhay korodhka PEI, iyo dhismayaasha siRNA/PEI oo leh saamiga lacag ka badan 16 waxay muujiyeen garaacid luciferase weyn oo ku jira khadka unugyada kansarka mindhicirka ee si joogto ah u muujinaya luciferase (unugyada Colon26 / Luc).Si kastaba ha ahaatee, cytotoxicity xoog leh iyo agglutination dhiigga ayaa lagu arkay siRNA/Lipofectamine complex iyo siRNA/PEI16 complex.Ku dallaca dhismayaasha cationic ee leh xarun anionic ayaa la sheegay inay tahay hab rajo leh oo lagaga gudbi karo suntan.Sidaa darteed waxaanu u diyaarinay dhismooyinka ternary ee siRNA oo leh PEI (qiimaynta saamiga 16) iyadoo lagu daro γ-PGA si loo yareeyo cytotoxicity loona bixiyo siRNA.Sida la filayo, cytotoxicity ee dhismayaasha ternary ayaa hoos u dhacay kororka maadada γ-PGA, taas oo hoos u dhigtay ζ-kartida dhismayaasha.Saamayn aamusnaan xoog leh oo la mid ah siRNA/Lipofectamine kaka ayaa laga helay dhismayaal ternary oo ay ku jiraan γ-PGA oo leh dulsaar anionic ah.Isku darka sare ee dhismooyinka ternary ee unugyada Colon26/Luc waxaa lagu xaqiijiyay microkoobiga fluorescence.Ka dib markii la gaadhay garaaca hidde-sidaha dibadda laga beddelay, awoodda isku-dhafan ee dhexdhexaadinta garaacista hidda-wadaha ilaalinta guriga, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), ayaa lagu qiimeeyay unugyada B16-F10.Isku-dhafka ternary (siRNA/PEI16/γ-PGA12 complex) ayaa muujiyay saamayn garaacid weyn oo GAPDH ah.Sidaa darteed, waxaan sameynay nidaam gaarsiinta siRNA oo faa'iido leh.