Alaabta

Wadooyinka molecular pathogenic ee cudurada beerka cirrhotic sida fayraska cagaarshow C (HCV), cagaarshow autoimmune (AIH) iyo biliary cirrhosis (PBC) ayaa si liidata loogu sifeeyay.Hidde-sideyaal kala duwan ayaa inta badan muhiim u ah cudur-sidaha.Isku-dhafka kala-goynta (SSH) waa hab-genome-ballaaran oo kobciya qoraallada mRNA ee si kala duwan loo muujiyey.Waxaan ujeednay inaan samayno indho-indheyn cusub oo muujinta hidda-wadaha kala duwan ee cirrhosis anagoo adeegsanayna SSH oo ay la socoto falcelinta silsiladda silsiladda polymerase ee dhabta ah (RT-PCR).Qoraallada beerka ee HCV cirrhosis, AIH cirrhosis, PBC, iyo unug beerka oo aan jirran ayaa baaray SSH.Natiijadii ka soo baxday DNA-ga kabka ah ee clone (cDNA) ayaa dib loo eegay si loo muujiyo kala duwanaanshiyaha isku-dhafka dhibcaha-blot ka dibna la isku xigxigay.Tilmaanta hidde-sidaha la xushay waxaa lagu qiyaasay RT-PCR-waqtiga-dhabta ah.Ka dib SSH, 694 clone ayaa dib loo eegay muujinta hidde-sidaha kala duwan, kuwaas oo 145 ka mid ah la isku xigxigay oo la ogaaday inay ka soo jeedaan 89 hiddo-sideyaal kala duwan.Todoba clones waxay ahaayeen kuwo isku mid ah oo kaliya oo leh taxanayaal la soo bandhigay (EST) taxane ah oo qeexaya hiddo-wadaha aan lahayn wax shaqo ah oo la yaqaan.Muujinta kor u qaadida ee afarta hiddo-wadaha ayaa lagu xaqiijiyay RT-PCR-waqtiga dhabta ah: transmembrane 4 superfamily 3 (tetraspanin CO-029) dhammaan noocyada cirrhosis, borotiinka isdhexgalka ee hedgehog (HIP) ee AIH cirrhosis iyo chitinase 3-like-1 (HC gp-39 ama ykl-40) iyo arginine-glutamic acid ku celis (RERE) ee HCV cirrhosis.RERE polymorphisms hiddo-wadaha iyo kala duwanaanshiyaha kala duwan ayaa lagu arkay dhammaan unugyada la baaray.Kor-u-qaadista Tetraspanin CO-029 ayaa ugu horreyntii lagu meeleeyay unugyada xuubka xabkaha.Gabagabadii, indho-indhayn cusub oo muujinta hidde-sidaha kala duwan ee cirrhosis-ka bini'aadamka ayaa la sameeyay iyadoo la isticmaalayo SSH oo ah aaladda daahfurka aasaasiga ah.Gaar ahaan, daraasado dheeraad ah oo ku saabsan hidda-wadaha RERE iyo alaabteeda ee HCV ee la xidhiidha cudurrada beerka waa la dammaanad qaaday.