Alaabta

Unugyada Eukaryotic waxay ka kooban yihiin dheelitirnaan jilicsan oo qadar daqiiqado ah oo ah afarta deoxyribonucleoside triphosphates (dNTPs), oo ku filan oo kaliya dhowr daqiiqo oo DNA ah.La'aanta iyo dheeraadka ah ee hal dNTP labaduba waxay keeni karaan kororka heerarka isbeddelka, hagaajinta DNA-da khaldan ama yaraanta mitokondrial DNA.dNTP-yada waxaa badanaa lagu qiyaasaa baaritaanka enzymatic kaas oo ku darista dATP shucaaca (ama shucaaca dTTP ee baaritaanka dATP) ee oligonucleotides synthetic oo gaar ah oo DNA polymerase ah ayaa u dhigma xoojinta dNTP aan la garanayn.Waxaan ogaanay in Klenow DNA-ga caadiga ah ee polymerase laga yaabo inuu beddelo ribonucleotide-ka u dhigma ee dNTP-ga aan la garanayn taasoo u horseedaysa xaaladaha qaarkood qiyaas weyn oo dNTPs ah.Waxaan hadda sharaxnay shuruudaha qiimeynta dNTP kasta ee ka fogaanaya ku darida ribonucleotide.Qiimaynta dTTP iyo dATP waxay ku filnaatay in la yareeyo uruurinta enzyme Klenow iyo kuwa lagu calaamadiyay dATP (ama dTTP);dCTP iyo dGTP waa in aan ku bedelno t he Klenow enzyme mid ka mid ah Taq DNA polymerase ama Thermo Sequenase.Waxaan soo jeedinaynaa in qaar ka mid ah warbixinadii hore ee ku darista ribonucleotide laga yaabo inay u keentay qiimayaal aad u sarreeya dGTP iyo dCTP.