Alaabta

CTP: fosfocholine cytidylyltransferase α (CCTα) waa ensaym nukliyeer ah kaas oo kicinaya tallaabada xaddidaadda xaddidaadda waddada CDP-choline ee isku-dhafka fosfatidylcholine (PC).Dhaqdhaqaaqa dufanka ee CCTα waxay keentaa in loo beddelo baqshadda nukliyeerka iyo ballaarinta shabakadda xuubabka gudaha ee loo yaqaan 'nucleoplasmic reticulum (NR)' iyadoo loo marayo hab ku lug leh qallafsanaanta xuubka.Laambadaha nukliyeerka ayaa sidoo kale looga baahan yahay xasiloonida iyo fidinta NR, laakiin haddii qaabkan gaarka ah, ama lamina nukliyeerka guud ahaan, looga baahan yahay PC-ga lama yaqaan.Si loo baadho xidhiidhkan, lamina nukliyeerka waxaa baabi'iyay RNAi ama carqaladeeyay muujinta Hutchinson-Gilford progeria syndrome (HGPS) mutant lamin A (progerin), iyo saamaynta CCTa iyo dheef-shiid kiimikaadka choline ayaa la falanqeeyay.Aamusnaanta siRNA-dhexdhexaadin ee lamin A/C ama lamin B1 ee unugyada CHO si loo yareeyo NR wax saameyn ah kuma yeelan isku-darka PC, halka laba jeer garaacid aan si gaar ah u xannibin waddada.Xaqiijinta doorkiisa yar ee isku-darka PC-ga, kaliya 10% ee choline/ethanolamine phosphotransferase si ku meel gaadh ah ayaa laga helay NR.Unugyada CHO, CCTα waxay ahayd nukleoplasmic waxayna la wadaageen GFP-progerin ee laalaabka nukliyeerka iyo galitaanka;si kastaba ha ahaatee, HGPS fibroblasts ayaa soo bandhigay qaybinta aan caadiga ahayn ee CCTα ee cytoplasm iyo baqshadda nukliyeerka taas oo ay la socoto hoos u dhigista 2-laab ee isku-darka PC.In kasta oo ay beddeshay deegaan ahaan, tijaabooyinka calaamadaynta choline-ku waxay muujiyeen in waxqabadka CCT aanu saamaynaynin, iyo xannibaadda isku-darka PC-ga ayaa la raacay hoos u dhaca dhaqdhaqaaqa hemicholinium-xasaasiyaha choline.Waxaan ku soo gabagabeyneynaa in CCTα iyo lamins ay si gaar ah u wada shaqeeyaan si loo sameeyo NR, laakiin qaabka guud ee baqshadda nukliyeerka ayaa saameyn yar ku leh dhaqdhaqaaqa CCT iyo isku dhafka PC.