BSA Cas: 9048-46-8 Budada cad ee la qaboojiyey ee la qaboojiyey
Lambarka buugaagta | XD90249 |
Magaca Alaabta | Serum Albumin |
CAS | 9048-46-8 |
Qaanuunka molecular | N/A |
Miisaanka Molecular | N/A |
Faahfaahinta Kaydinta | 2 ilaa 8°C |
Xeerka Tarifka La Iswaafajiyay | 35029070 |
Tilmaamaha Alaabta
Wka dib | 5.0% ugu badnaan |
Kaydinta | Ku hay meel qabow, qalalan, meel mugdi ah |
Muuqashada | budo cad |
Wadarta waxa ku jira borotiinka (tijaabada Biuret) | 98% min |
Nadiifinta BSA ee borotiinka (Electrophoresis test) | 96% min |
Solubility (10% gudaha H2O) | 15 |
pH (5% biyaha ku jira) | 6.5 - 7.4 |
OD403nm (1% gudaha H2O) | 0.15% ugu badnaan |
Isticmaalka cilmi-baarista oo keliya, maaha isticmaalka aadanaha | cilmi baadhida loo isticmaalo oo kaliya, ee aan loo isticmaalin bini aadamka |
Hordhac: BSA waa mid ka mid ah borotiinada inta badan loo isticmaalo shaybaarada kiimikaalka, waxaana laga yaabaa in muhiimaddeeda lagu ilduufo tijaabooyinka sababtoo ah waa mid aad u badan oo aad u badan.Serum albumin (BSA), oo sidoo kale loo yaqaano qaybta shanaad, waa globulin ku jirta serum bovine oo ka kooban 583 amino acid hadhaa, oo leh miisaan kelli ah 66.430kDa iyo barta isoelectric ee 4.7.BSA waxay leedahay codsiyo kala duwan oo kala duwan oo ku saabsan tijaabooyinka kiimikaad, sida wakiilka xannibaadda ee galbeedka.
Codsiga: Serum Albumin (BSA), oo sidoo kale loo yaqaano qaybta shanaad, waa globulin ku jirta serum bovine, oo ka kooban 607 amino acid haraaga, waxayna leedahay codsiyo kala duwan oo tijaabo ah oo kiimikaad ah.Serum albumin-ka Bovine waxaa guud ahaan loo isticmaalaa sidii xasiliye ee xalka kaydinta iyo xal-celinta falcelinta enzymes-ka xaddidan ama enzymes la beddelay si loo xasiliyo dhaqdhaqaaqa enzyme loogana hortago kala-goynta enzyme iyo xayeysiis aan gaar ahayn.
Shaqada: BSA waxaa guud ahaan loo isticmaalaa sidii xasiliye ee xalka kaydinta iyo xalalka falcelinta enzymes xaddidaadda ama enzymes la beddelay, sababtoo ah enzymesyada qaarkood waa kuwo aan degganayn ama waxay leeyihiin firfircooni hooseeya marka loo eego uruurinta hoose.Ka dib marka lagu daro BSA, waxay ciyaari kartaa doorka "ilaalinta" ama "sidee", iyo waxqabadka enzymes badan ayaa si weyn loo wanaajin karaa ka dib marka lagu daro BSA.Enzymes aan u baahnayn in lagu daro BSA guud ahaan ma saamayso ku darida BSA.Inta badan DNA substrate-ka, BSCA waxay ka dhigi kartaa dheefshiidka mid aad u dhameystiran, waxayna gaari kartaa goyn soo noqnoqda.Markay tahay 37°C, marka falcelinta dheefshiidku dhaafo 1 saac, BSA waxay ka dhigi kartaa ensaymka mid xasiloon, sababtoo ah falcelinta falcelinta aan lahayn BSA, enzymes xaddidan oo badan ayaa kaliya ku noolaan kara 37 ° C 10 ~ 20min ama xitaa waqti gaaban..Taas bedelkeeda, BSA waxay ku xidhi kartaa ion birta iyo kiimikooyinka kale ee kaydinta ama substrate DNA ee xannibaya dhaqdhaqaaqa xaddidaadda endonucleases.
Adeegsada: Heerka caadiga ah ee serum albumin (BSA, StandardGrade), kaas oo dabooli kara baahida tijaabooyinka joogtada ah, sida wakiilka difaaca jirka, unugyada unugyada (xayawaanka bakteeriyada iyo unugyada cayayaanka, iwm.) nafaqooyinka dhaqanka iyo qaybaha dhaqanka, xasilinta borotiinka/ensamiga reagents iyo heerarka qiyaasta borotiinka.Heerka ogaanshaha serum albumin (BSA, DiagnosticGrade) waxa uu dabooli karaa inta badan baahiyaha tijaabada ah ee joogtada ah, sida wakiilka difaaca jirka, borotiinka/dejinta enzyme, dareeraha, side iyo heerka borotiinka.Intaa waxaa dheer, waxa kale oo loo isticmaali karaa immunoassays, dhaqanka unugyada iyo tijaabo hybridization oo u baahan dareen sare.