Alaabta

Bicine waa kaydka amino acid zwitterionic, oo firfircoon pH 7.6-9.0 (pKa of 8.26 at 25°C).Badbaadinta lagu taliyay ee heerkulka hoose ee shaqada biochemical.Bicine waxaa loo isticmaalaa diyaarinta xal substrate deggan si loo go'aamiyo serum guanase.Isticmaalka bicine ee lakabka khafiifka ah ee ion beddelka habka chromatography ee xallinta borotiinka ayaa la daabacay.Bicine ayaa loo adeegsaday peptide iyo crystallization protein.Daraasad kinetic ah oo ku saabsan isku-dhafka afar-geesoodka ah ee isku-dhafka analogga ah ee creatine kinase ayaa loo adeegsaday bicine kaydiyaha falcelinta.Nidaam dhawr waji leh oo loogu talagalay SDS-PAGE ee borotiinnada iyo peptides ee ku jira bicine ayaa lagu sifeeyay.

Darbiyada alveolar ee keligood ah ayaa lagu sameeyay daraasado dhaadheer oo kacsan oo cusbo ah iyo Bicine (0.2 M) waxay maraan suuska sii socda ee xiisadda unugyada (TTD).Waxaan baarnay saameynta xalalka kala duwan ee TTD-gan waxaanan raadinnay isbeddello u dhigma qaab-dhismeedka ultrastructure.Parenchyma sambabada ayaa lagu kala jeexjeexay hal darbi oo alveolar ah (30 X 30 X 150 microns) ee cusbada fosfat-buffered (0.15 M).Waxaa loo wareejiyay qubeyska dhererka kacsanaanta, nudaha waxaa lagu dhex milmay Bicine, saline, Xalka Hank ee la xoojiyay, 0.25% Alcian blue ee salineed, ama sodium dodecyl sulfate xal, muddooyin kala duwan.Wareegtada lagu sameeyay kordhinta la bixiyay oo xoogga ugu sarreysa lagu qiyaasay waqti ka dib, isla unugyadaan ayaa lagu hagaajiyay glutaraldehyde/tannic acid buffered waxaana laga baaraandegay microscopy elektaroonig ah.Darbiyada alveolar ee keliga ah ee lagu dhex milmay milix ama Bicine waxay muujiyeen TTD horumarsan.Vacuoles ama meelo banaan ayaa ka soo muuqday interstitium kaas oo habacsanaan unuggu uu ku hormaray TTD.Lagu arkay 0.3 saacadood gudahood, isbeddellada ayaa si wanaagsan u horumaray 0.6 saac.Si kastaba ha ahaatee, sodium dodecyl sulfate (70 mM), si kastaba ha ahaatee, ma jirin TTD iyo qaab-dhismeed ahaan ma jirin interstitium, iyada oo kaliya xuubabka hoose iyo borotiinnada fibreska ah.Xalka Hank ee la xoojiyey ama 0.25% Alcian buluug, matrix interstitial, qaab-dhismeedka unugyada iyo xiisadda unugyada ayaa si wanaagsan loo ilaaliyay 1 saac.Daraasadani waxay soo jeedinaysaa in dheecaanka matrixka interstitial uu ku dhaco saline ama Bicine, iyo matrix aan sax ahayn ayaa lagama maarmaan u ah ilaalinta xiisadda unugyada.