Alaabta

APS-5 waa substrate chemiluminescent ah oo ku salaysan 9,10-dihydroacridine, taas oo inta badan loo isticmaalo ogaanshaha ELISA ee xeryahooda leh phosphatase alkaline AP conjugates;9,10-dihydroacridine ee qaab dhismeedka APS-5 Qaab dhismeedka hydroacridine wuxuu iftiimin karaa iftiinka si joogto ah, si deggan oo hufan hoos yimaada ficilka AP, iyo APS-5 waxay aad ugu habboon tahay baaritaanka xalinta firfircoonida fosfatase iyo fosfatase enzyme-ku xiran immunoassay.

Sida baaritaanka luminescent, waxaa loo isticmaalaa daraasadda jajabyada hiddaha.Dareen-celinta waxa lagu calaamadeeyay acridan (9,10-dihydroacridine) sida substrate iyo fosfatase alkaline, taasoo soo saarta chemiluminescence-xoog sare oo joogto ah.Waxay siisaa dareenka sare iyo fududaynta isticmaalka isku xidhka fosfatase alkaline inta lagu jiro ogaanshaha kiimikaalka.Fosphatase alkaline waxaa lagu ogaadaa in ka yar 10-19 mol, si degdeg ah ayuu u sarreeyaa si loo yareeyo waqtiga ogaanshaha iyo kordhinta wax soo saarka, iyo jiirada qalooca toosan ee toosan waxaa lagu sawiray logarithmic siman 1.0.Hal tiro ama in ka badan oo ensaymes ah waxay soo saartaa hal cadad ama in ka badan oo iftiin leh, iftiin joogto ah - oo aan aad u baahnayn waqtiga qiimaynta.Xoogagga iftiinka iftiinka waxaa laga akhrin karaa qalooca toosan ee toosan ee abuurmay wakhti kasta, iyo natiijooyinka lafa-gurka waa dareen la'aan heerkulka u dhexeeya 22 ° C - 35 ° C, yaraynta saxnaanta loo baahan yahay si loo xakameeyo heerkulka.

faa'iido:

1 Dareen sare - calaamadaha borotiinka ee ka hooseeya 5pg waa la ogaan karaa;

2 Xoogga iftiinka sare - iftiinka iftiinka waxaa lagu gaari karaa waqti gaaban;

3 luminescence joogto ah oo xasilan - geeddi-socodka luminescence-keeda ma saameynayo heerkulka 25-35 ℃, uma baahna xakameynta heerkulka.

Isbarbardhigga arjiga ee substrate-ka iftiinka iftiinka ee sida caadiga ah loo isticmaalo APS-5 (193884-53-6) iyo AMPPD (122341-56-4)

Labada APS-5 (193884-53-6) iyo AMPPD (122341-56-4) ayaa ah kiimikooyin kimiluminescent ah oo loogu talagalay fosfatase alkaline, labadaba waxaa loo yaqaan reagents ogaanshaha aadka u sarreeya, markaa labadan reagents waxay ku jiraan ogaanshaha Kee fiican iyo kee baa ka daran?Sidee loo doortaa marka la isticmaalayo?

Dhinaca kale, marka APS-5 loo isticmaalo sida substrate-ka-helitaanka fosfatase alkaline, dareenka ogaanshaha wuxuu gaari karaa 10-19 xoojinta molar, wakhtiga horumarinta midabku wuxuu gaari karaa heerka ugu sarreeya ee ku saabsan 10 ilbiriqsi, iyo fluorescence wuxuu soconayaa muddo dheer. waqti.Dhinacyadan, APS 5 waxa la odhan karaa waxay leedahay sifooyin aad u wanaagsan.Si kastaba ha ahaatee, APS-5 waxay ku xaddidan tahay heerkulka codsiga, iyo heerkulka samaynta midabka ayaa doorbidaya in ka hooseeya 20'C.Dhanka kale, marka AMPPD loo isticmaalo sida substrate-ka ogaanshaha fosfatase alkaline, dareenka ogaanshaha waxaa sidoo kale loo yaqaan dareenka ultra-sare, laakiin ma gaari karo heerka APS 5. Waqtiga horumarinta midabku wuxuu gaaraa heerka ugu sarreeya qiyaastii 30 ilbiriqsi , iyo fluorescence muddada dheer.AMPPD aad ayay ugu fiican tahay guryaha sare, laakiin asal ahaan way ka hoosaysaa APS5, laakiin heerkulkeeda midabaynta maaha mid adag sida APS5, heerkulka kaydinta 2--8C wuu ka sahlan yahay APS-5.Marka la soo koobo, in kasta oo APS-5 ay ka sarreyso AMPPD saxnaanta ogaanshaha, waxay luminaysaa xasilloonida.Sida loo doorto waxay kuxirantahay baahida gaarka ah ee ogaanshaha.

1. Horudhac APS-5 (193884-53-6):

Magaca Ingiriisiga: APS-5

Lambarka CAS: 193884-53-6

Qaaciddada molecular: C21H15ClNO4PS Na2

Miisaanka molecular: 489.819

Muuqaal: budo adag oo cad-cad

Waxa ku jira: ≥98%

Tilmaamaha: 1g, 5g, 10g

Xaaladaha kaydinta: -20 ℃ ilaalinta

(4-Chlorophenylmercapto) (10-methyl-9,10-dihydroacridine methylene) phosphate disodium milix, oo loo yaqaan APS-5, waa reagent chemiluminescent waxaana loo isticmaalaa sidii substrate kiimiluminescent ah.Waxay siisaa dareenka sare iyo fududaynta isticmaalka isku xidhka fosfatase alkaline inta lagu jiro ogaanshaha kiimikaalka.Dareen-celinta waxa lagu calaamadeeyay acridan (9,10-dihydroacridine) sida substrate iyo fosfatase alkaline, taasoo soo saarta chemiluminescence-xoog sare oo joogto ah.APS-5 waa falcelis ku habboon go'aaminta waxqabadka fosfatase iyada oo la adeegsanayo qiimeynta xalalka iyo phosphatase enzyme-ku xiran immunoassays.Sida substrate luminescent, APS-5 waxay leedahay dareen ogaansho sare leh iyo waqti falcelin gaaban.

2. Soo bandhigid faahfaahsan ee AMPPD (122341-56-4)

Magaca Ingiriisiga: AMPPD

CAS No: 122341-56-4

Qaaciddada molecular: C18H23O7P

Miisaanka molecular: 382.344781

Muuqaal: budo cad

Daahirsanaanta: ≥97%

Tilmaamaha: 1g, 5g, 10g

Xaaladaha kaydinta: -20 ℃ ilaalinta

Taxadir: Waa in ay ahaato mid qallalan si looga fogaado kala diris badan iyo lyophilization