Reagent-ka cusub ee Trinder waa derivative aniline oo aad u biyo-milmi kara kaas oo si weyn loogu isticmaalo baadhista ogaanshaha iyo baadhista biochemical.Waxay leedahay faa'iidooyin dhowr ah marka loo eego reageniyeyaasha chromogenic caadiga ah ee go'aaminta midabka midabka ee dhaqdhaqaaqa hydrogen peroxide.Reagement-ka cusub ee Trinder ayaa si ku filan u xasilan labadaba Waxaa loo isticmaali karaa xalka ama nidaamka ogaanshaha dhuumaha tijaabada.Marka ay joogaan hydrogen peroxide iyo peroxidase, reagent Trinder'si cusub ayaa loo isticmaali karaa iyadoo lagu darayo 4-aminoantipyrine (4-AA) ama 3 methyl Inta lagu jiro falcelinta isku dhafka oksaydhka ee benzothiazole sulfonehydrazone (MBTH), midabyo guduudan ama buluug ah oo aad u deggan. la sameeyo.Nuugista xuubka dheeha ee ku lammaan MBTH waa 1.5-2 jeer ka sarreeya kuwa midabada leh ee la socda 4-AA;si kastaba ha ahaatee, 4-AA Xalku wuu ka xasilloon yahay xalka MBTH.Substrate-ka waxaa enzymatically oksaydhed by oksaydhistiisa si ay u abuuraan hydrogen peroxide.Xoojinta hydrogen peroxide waxay u dhigantaa fiirsashada substrate-ka.Sidaa darteed, qadarka substrate-ka waxaa lagu go'aamin karaa horumarinta midabka falcelinta isku-xidhka oksaydhka.Glucose, aalkolada, acyl-CoA, iyo kolestaroolka ayaa loo isticmaali karaa in lagu ogaado substrate-kaas oo ay weheliyaan novel Trinder's reagents iyo 4-AA.Waxaa jira 10 cusub oo Trinder reagents ah.Ka mid ah kuwa cusub ee Trinder's, TOOS waa tan inta badan la isticmaalo.Si kastaba ha noqotee, substrate gaar ah, tijaabinta noocyada kala duwan ee reagents-ga cusub ee Trinder ayaa lagama maarmaan u ah horumarinta nidaamka ogaanshaha ugu fiican.
