Hiddo-sidaha beta-glucosidase (bgl3) oo ka yimid Streptomyces sp.QM-B814 (Ururinta Dhaqanka Nooca Ameerikaanka 11238) waxa lagu xidhay dhamaystirka shaqaynta ee beta-glucosidase-negative mutant ee Streptomyces lividans.Qaab akhris-furan oo ah 1440 nucleotides oo deddejinaya polypeptide oo ah 479 amino acids ayaa la helay iyadoo la raacayo.Borotiinka qarsoon (Bgl3) wuxuu muujinayaa isu ekaanshaha ballaaran (in ka badan 45% aqoonsiga) oo leh beta-glycosidases oo ka yimid qoyska-1 glycosyl hydrolases.Enzyme-ka la kariyey, oo la nadiifiyey ka dib roobabka ammonium sulphate iyo laba tillaabo chromatographic, waa monomeric leh cufnaanta molecular 52.6 kDa, sida lagu go'aamiyo spectrometry mass, iyo barta isoelectric ee pI 4.4.Insaymku wuxuu u muuqdaa inuu yahay beta-glucosidase oo leh gaar ahaan substrate ballaadhan, wuxuu ku firfircoon yahay celloligomers, wuxuuna sameeyaa falcelinta transglycosylation.Qiyaasta Km ee muuqata ee p-nitrophenyl-beta-D-glucopyranoside iyo cellobiose waa 0.27 mM iyo 7.9 mM, siday u kala horreeyaan.Ki wuxuu ku qiimeeyaa gulukoosta iyo delta-gluconolactone, iyadoo la isticmaalayo p-nitrophenyl-beta-D-glucopyranoside substrate ahaan, waa 65 mM iyo 0.08 mM, siday u kala horreeyaan.Insaymka la safeeyey ayaa leh pH ugu wanaagsan ee pH 6.5 heerkulka ugu fiican ee dhaqdhaqaaqa waa 50 darajo
