Alaabta

1.Waxaan ku barannay saameynta xannibaadda ee isorhamnetin ee likaha tyrosinase iyadoo la xakameynayo kinetics iyo jilitaanka xisaabinta.Isorhamnetin waxay dib u celisay tyrosinase qaab isku dhafan oo ah Ki=0.235±0.013 mM.Cabbiraadaha gudaha iyo 1-anilinonaphthalene-8-sulfonate(ANS) -isku-xidhka fluorescence waxay muujisay in isorhamnetin aanu u keenin isbeddello la taaban karo oo ku yimaadda qaab-dhismeedka jaamacadda ee tyrosinase.Si loo helo aragti ku saabsan habka hawlgelinta, kinetics-ka waxaa lagu xisaabiyay cabbirro dhex-dhexaad ah iyo falcelinta substrate-ka joogtada ah.Natiijooyinka waxay muujiyeen in firfircooni la'aanta ay keentay isorhamnetin ay ahayd falcelin-dalab-kii ugu horreeyay ee hababka laba-cirifoodka.Si aan u helno fahan dheeri ah, waxaanu ku ekaynay xidhidhiyaha u dhexeeya tyrosinase iyo isorhamnetin.Jilitaanka ayaa lagu guuleystay (tamar ku xiran Dock6.3: -32.58 kcal/mol, AutoDock4.2: -5.66 kcal/mol, iyo Fred2.2: -48.86 kcal/mol), taasoo soo jeedinaysa in isorhamnetin ay la falgasho dhowr hadhaaga, sida sida HIS244 iyo MET280.Istaraatiijiyadan isdhexgalka tyrosinase ee saadaasha ah oo ay weheliso kinetics oo ku salaysan xarun flavanone ayaa laga yaabaa inay faa'iido u yeelato baaritaanka tyrosinase dabiiciga ah ee suurtagalka ah.

2.Acid dariiqa furfuran ee conalbumin (CA), monomeric glycoprotein ka digaaga ukunta cad, ayaa lagu baadhay iyadoo la isticmaalayo fog-iyo dhow-UV CD spectroscopy, qiiqa fluorescence gudaha intrinsic fluorescence probe 1-anilino-8-napthalene sufonate (ANS) iyo kala firdhinta iftiinka firfircoon (DLS).Waxaan aragnaa isbeddelada pH-ku-tiirsan ee qaabdhismeedka sare iyo kan sare ee CA.Waxay leedahay qaab dhismeed sare oo u eg α-helical oo ah pH 4.0 laakiin qaab dhismeedka luminta pH 3.0.CA waxay si gaar ah ugu jirtay sidii xaalad globule ka hor dhalaalaysa iyo dawlad dhalaalaysa oo ah xal pH 4.0 iyo pH 3.0, siday u kala horreeyaan.Saamaynta pH ee is-waafajinta iyo heerkulbeegga CA waxay tilmaamaysaa caabbinta kulaylka ee pH dhexdhexaad ah.Natiijooyinka DLS waxay muujinayaan in gobolka MG uu u jiray qaab is haysta oo ah xalal biyo leh oo leh radis hydrodynamic ee 4.7 nm.Deminta fluorescence tryptophan by acrylamide waxay sii xaqiijisay isu-ururinta dawlad dhexe, oo qayb ka mid ah, oo u dhaxaysa dawladaha waddaniga ah iyo kuwa furfuran.