Alaabta

Fosforyaalka borotiinku wuxuu nidaamiyaa shaqooyinka unugga ee kala duwan wuxuuna door muhiim ah ka ciyaara horumarinta hore ee dhirta.Si loo dhamaystiro loona balaadhiyo baadhitaanadii hore ee fosfooraska borotiinka ee geedo Arabidopsis waxaanu isticmaalnay habab kale oo isku daraya soo saarista borotiinka ee ku jira xaalado aan denaturing lahayn oo ay weheliso chromatography bir-ion affinity chromatography (IMAC) ee kobcinta fosfooprotein aan sax ahayn oo laga soosaaray Rubisco, oo ay ku xigto aqoonsi iyadoo la isticmaalayo laba-cabbir jel electrophoresis (2-DE) iyo dareeraha chromatography-tandem mass spectrometry (LC-MS/MS).Dheefshiidka trypsin-in-gel iyo falanqaynta dhibcaha jel ee la doortay ayaa lagu aqoonsaday 144 fosfooraska peptides iyo hadhaaga, kuwaas oo kaliya 18 fosphopeptides iyo 8 phosphosites laga helay PhosPhAt 4.0 iyo P3DB Arabidopsis thaliana phosphorylation databases.In ka badan kala badh 82-kii fosfooprotein ee la aqoonsaday ayaa ku lug lahaa dheef-shiid kiimikaad karbohaydrayt ah, sawir-qaadista/neefsashada ama ka jawaab celinta cadaadiska oksaydhka ee makaanigga.Kobcinta fosforootiinnada saxda ah ka hor 2-DE iyo LC-MS/MS waxay u muuqataa inay kor u qaadayso ogaanshaha fosfooraska threonine iyo tyrosine marka la barbar dhigo hababka loo isticmaalo kobcinta heerka peptide, taasoo soo jeedinaysa in labada hab ay yihiin kuwo is-kaabaya marka la eego daboolida goobta fosfooraska.Isbarbardhigga natiijooyinka geedo yaryar iyo kuwa hore loo helay caleemaha Arabidopsis baaluq ayaa lagu aqoonsaday shan borotiino kuwaas oo si kala duwan u leh fosforyaalka unugyadaas, taas oo muujinaysa awoodda farsamadan ee lagu baadho dhaqdhaqaaqa fosforyaalka borotiinka inta lagu jiro horumarinta dhirta.