Laba isomeric bicyclo [4.1.0] heptane analogues ee glycosidase inhibitor galacto-validamine, (1R *,2S,3S,4S,5S,6S*) -5-amino-1- (hydroxymethyl) bicyclo[4.1.0] heptane -2,3,4-triol, ayaa lagu soo saaray 13 tilaabo laga soo bilaabo 2,3,4,6-tetra-O-benzyl-D-galactose.Dhaqdhaqaaqyada xannibaadda ee labada amines-ka kooban ee xaddidan, iyo acetamides-ka u dhigma, ayaa lagu cabbiray ganacsiga alfa-galactosidase enzymes ee ka soo baxa digirta qaxwada iyo E. coli.Dhaqdhaqaaqa glycosyl hydrolase qoyska GH27 enzyme (digirta qaxwaha) waxaa si tartan ah u hakiyay 1R,6S-amine (7), isdhexgalka isku xiran kaas oo lagu gartey qiimaha K (i) ee 0.541 microM.GH36 E. coli alpha-galactosidase waxay soo bandhigtay isdhexgal xidhidh aad u daciif ah oo leh 1R,6S-amine (IC(50)= 80 microM).The diastereomeric 1S,6R-amine (9) si daciif ah ugu xidhan labada galactosidases, (digirta qaxwaha, IC(50)= 286 microM) iyo (E. coli, IC(50)= 2.46 mM).
