γ-L-Glutamyl-3-carboxy-4-nitroanilide, cusbada monoammonium Cas:63699-78-5 budada microcrystalline huruud ah
Lambarka buugaagta | XD90183 |
Magaca Alaabta | γ-L-Glutamyl-3-carboxy-4-nitroanilide, cusbada monoammonium |
CAS | 63699-78-5 |
Qaanuunka molecular | C12H12N3O7-.H4N+ |
Miisaanka Molecular | 328.27 |
Faahfaahinta Kaydinta | Ambient |
Tilmaamaha Alaabta
Muuqashada | budada microcrystalline huruud khafiif ah |
Asay | 99% |
barta dhalaalaysa | 187ºC ± 2.0ºC |
Barta karkarinta | 718.3°C at 760mmHg |
Barta toosan | 368.5C |
Solubility | H2O: 100 mg/ml, cad, jaalle-cagaaran |
Kinetics ee serum gamma-glutamyltransferase (EC 2.3.2.2) ayaa la baadhay, iyadoo la isticmaalayo glycylglycine sida gamma-glutamyl substrate gamma-glutamyl iyo gamma-glutamyl-4-nitroanilide iyo derivative-ka carboxy, gamma-glutamyl-3-carboxy-4 -nitroanilide, sida ku-deeqaha substrates.Dhacdada isku mar ah ee gamma-glutamyltransfer iyo autotransfer labadaba waxaa lagu aasaasay chromatography waraaqaha.Sirqoollada is-dhaafsiga joogtada ah ee saamiga laba-is-dhaafsiga ah waxay xaqiijinayaan in habka ensaymku uu yahay mid aan xidhiidh lahayn (ping-pong bi-bi).Joojinta ku-deeqaha midkoodna lama helin, iyo xannibaadda glycylglycine ayaa lagu arkay oo keliya meelaha ka sarreeya kuwa danta bukaan-socodka.Joogtada Kinetic ee lagu helay falanqaynta dib-u-celinta aan tooska ahayn ee xogta xawaaraha bilowga ah ayaa loo isticmaalay si loo go'aamiyo uruurinta substrate-ka reagent ee baaritaanka ensaymkan.Qiimaynta isticmaalka 4 mmol ee gamma-glutamyl-3-carboxy-4-nitroanilide iyo 100 mmol ee glycylglycine litirkiiba waxa ay keentay hawlo u dhigma kuwa lagu qiyaaso 4 mmol ee gamma-glutamyl-4-nitroanilide iyo 40 mmol oo glycylglycine halkii litir.Xaddigan ku-deeqaha karboksijiinta iyo glycylglycine sidoo kale waa "qiimaha ugu fiican" mana keenaan dhibaatooyin habraac ah marka la isticmaalo.