X-GAL CAS: 7240-90-6 98% Caddaan ilaa caddaan ka baxsan budada Crystalline
| Lambarka buugaagta | XD90008 |
| Magaca Alaabta | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Qaanuunka molecular | C14H15BrClNO6 |
| Miisaanka Molecular | 408.63 |
| Faahfaahinta Kaydinta | -2 ilaa -6 °C |
| Xeerka Tarifka La Iswaafajiyay | 29400000 |
Tilmaamaha Alaabta
| Muuqashada Xalka | Cad, aan midab lahayn ama xal huruud ah khafiif ah (50mg/ml gudaha DMF:MeOH, 1:1) |
| Wareegga indhaha ee gaarka ah | -61.5 +/- 1 |
| Muuqashada | Caddaan ama budo crystalline ah |
| Daahirnimada HPLC | min 99% |
| Solubility (5% gudaha DMF) | milmay (5% w/v,DMF) |
| Biyaha KF | ugu badnaan 1% |
| Assay (HPLC oo ku salaysan Anhydrous) | min 98% w/w |
Isticmaalka X-gal
X-gal (sidoo kale loo soo gaabiyo BCIG ee 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) waa unug dabiici ah oo ka kooban galactose oo ku xiran indole la beddelay.Xarunta waxaa sameeyay Jerome Horwitz iyo la-hawlgalayaashii 1964. Magaca rasmiga ah ee kiimikaad ayaa badanaa la soo gaabiyaa si aan sax ahayn laakiin sidoo kale weedho dhib yar sida bromochloroindoxyl galactoside.X ka soo baxa indoxyl ayaa laga yaabaa inuu noqdo isha X ee foosha X-gal.X-gal waxaa badanaa loo isticmaalaa bayoolaji molecular si loo tijaabiyo joogitaanka enzyme, β-galactosidase, meesha bartilmaameedka caadiga ah, β-galactoside.Waxa kale oo loo isticmaalaa in lagu ogaado dhaqdhaqaaqa enzyme this ee histochemistry iyo bakteeriyada.X-gal waa mid ka mid ah indoxyl glycosides iyo esters badan oo soo saara xeryahooda buluuga ah ee aan la milmi karin oo la mid ah dheeha indigo taas oo ay sabab u tahay hydrolysis-catalyzed enzyme.
X-gal waa analooga lactose ah, sidaas darteed waxaa laga yaabaa in lagu nadiifiyo β-galactosidase enzyme kaas oo kala gooya bond β-glycosidic ee D-lactose.X-gal, marka ay kala jeexdo β-galactosidase, waxay soo saartaa galactose iyo 5-bromo-4-chloro-3-hydroxyindole - 1. Kan dambe si iskiis ah ayuu u yaraadaa oo waxaa loo sameeyaa 5,5'-dibromo-4,4'-dichloro -indigo - 2, badeeco buluug ah oo aad u daran oo aan la milmi karin.X-gal lafteedu waa mid aan midab lahayn, markaa joogitaanka badeecada midabka buluuga ah waxaa laga yaabaa in loo isticmaalo tijaabo ahaan joogitaanka β-galactosidase firfircoon.Tani waxay sidoo kale u oggolaaneysaa bakteeriyada β-galactosidase (oo loo yaqaan lacZ) in loo isticmaalo wariye ahaan codsiyo kala duwan.
Falanqaynta laba-isku-dhafka ah, β-galactosidase waxaa loo isticmaali karaa wariye ahaan si loo aqoonsado borotiinnada is-dhexgalka midba midka kale.Habkan, maktabadaha genome-ga waxaa laga yaabaa in lagu baaro isdhexgalka borotiinka iyadoo la isticmaalayo nidaamka khamiirka ama bakteeriyada.Meesha ay jirto is dhexgalka guusha leh ee ka dhexeeya borotiinnada la baaray, waxay keeni doontaa in lagu xidho xayndaabka hawlgeliyaha.Haddii dhiirigeliyaha uu ku xiran yahay hidda-wadaha lacZ, soo-saarka β-galactosidase, taas oo keenta sameynta gumeysi buluug-buluug ah oo ku jira joogitaanka X-gal, sidaas darteed waxay muujin doontaa isdhexgalka guusha u dhexeeya borotiinnada.Farsamadan waxa laga yaabaa inay ku koobnaato maktabadaha baadhista oo cabbirkoodu ka yar yahay ku dhawaad 106. Kala-jaridda guusha leh ee X-gal waxay sidoo kale abuurtaa ur xun oo la dareemo taasoo ay ugu wacan tahay isbedbeddelka indole.
Maadaama X-gal lafteedu aanu midab lahayn, joogitaanka badeecada midabka buluuga ah waxa loo isticmaali karaa tijaabo ahaan joogitaanka β-galactosidase firfircoon.
Aqoonsigan fudud ee ensaymka firfircoon wuxuu u oggolaanayaa hidda-wadaha βgalactosidase ( hidda-wadaha lacZ ) in loo isticmaalo hiddo-wadaha wariyaha ee codsiyada kala duwan.
