Alaabta

Dareeraha biyo-milmi kara ee go'aaminta photometric enzymatic ee hydrogen peroxide.Dib-u-hawlgalayaasha Trinder-ka cusub ayaa ah kuwa aadka u biyo-milmi kara ee aniline-ka kuwaas oo si weyn loogu isticmaalo baaritaannada ogaanshaha iyo baarista biochemical.Waxaa jira faa'iidooyin dhowr ah oo ka sarreeya reageniyeyaasha chromogenic caadiga ah ee go'aaminta midabka midabka ee dhaqdhaqaaqa hydrogen peroxide.Dib-u-hawlgalayaasha Trinder-ka cusub waa kuwo deggan oo ku filan in loo isticmaalo xalka iyo hababka ogaanshaha dhuumaha tijaabada labadaba.Joogitaanka hydrogen peroxide iyo peroxidase, reagent-ka cusub ee Trinder's reagent ayaa la muujiyay inuu ka falceliyo 4-aminoantipyrine (4-AA) ama 3-methylbenzothiazole sulfonehydrazone (MBTH) inta lagu guda jiro falcelinta isku-xidhka oksaydhka.Waxay samaysaa midabyo buluug ah ama buluug ah.Nuugista xuubka dheeha ee ku lammaan MBTH wuxuu ahaa 1.5-2 jeer ka sarreeya tan dheeha oo ay weheliso 4-AA;si kastaba ha ahaatee, xalka 4-AA wuxuu ahaa mid ka xasilloon marka loo eego xalka MBTH.Substrate-ka waxaa enzymatically oksaydhed by oxidase ay si ay u soo saaraan hydrogen peroxide.Fogaanta hydrogen peroxide waxay u dhigantaa xoojinta substrate-ka.Sidaa darteed, qadarka substrate-ka waxaa lagu go'aamin karaa horumarinta midabka falcelinta isku-xidhka oksaydhka.Glucose, aalkolada, acyl-CoA iyo kolestaroolka ayaa loo isticmaali karaa in lagu ogaado substrate-kaas oo ay weheliyaan novel Trinder's reagent iyo 4-AA.Waxaa jira 10 cusub oo Trinder's reagents ayaa diyaar ah.Ka mid ah kuwa cusub ee Trinder's, TOOS waa tan inta badan la isticmaalo.Si kastaba ha ahaatee, substrate gaar ah, tijaabinta fasalada kala duwan ee reagents-ga cusub ee Trinder ayaa lagama maarmaan u ah horumarinta nidaamka ogaanshaha ugu fiican.

Dareeraha biyo-milmi kara ee go'aaminta photometric enzymatic ee hydrogen peroxide.Dib-u-hawlgalayaasha Trinder-ka cusub ayaa ah kuwa aadka u biyo-milmi kara ee aniline-ka kuwaas oo si weyn loogu isticmaalo baaritaannada ogaanshaha iyo baarista biochemical.Waxaa jira faa'iidooyin dhowr ah oo ka sarreeya reageniyeyaasha chromogenic caadiga ah ee go'aaminta midabka midabka ee dhaqdhaqaaqa hydrogen peroxide.Dib-u-hawlgalayaasha Trinder-ka cusub waa kuwo deggan oo ku filan in loo isticmaalo xalka iyo hababka ogaanshaha dhuumaha tijaabada labadaba.Joogitaanka hydrogen peroxide iyo peroxidase, reagent-ka cusub ee Trinder's reagent ayaa la muujiyay inuu ka falceliyo 4-aminoantipyrine (4-AA) ama 3-methylbenzothiazole sulfonehydrazone (MBTH) inta lagu guda jiro falcelinta isku-xidhka oksaydhka.Waxay samaysaa midabyo buluug ah ama buluug ah.Nuugista xuubka dheeha ee ku lammaan MBTH wuxuu ahaa 1.5-2 jeer ka sarreeya tan dheeha oo ay weheliso 4-AA;si kastaba ha ahaatee, xalka 4-AA wuxuu ahaa mid ka xasilloon marka loo eego xalka MBTH.Substrate-ka waxaa enzymatically oksaydhed by oxidase ay si ay u soo saaraan hydrogen peroxide.Fogaanta hydrogen peroxide waxay u dhigantaa xoojinta substrate-ka.Sidaa darteed, qadarka substrate-ka waxaa lagu go'aamin karaa horumarinta midabka falcelinta isku-xidhka oksaydhka.Glucose, aalkolada, acyl-CoA iyo kolestaroolka ayaa loo isticmaali karaa in lagu ogaado substrate-kaas oo ay weheliyaan novel Trinder's reagent iyo 4-AA.Waxaa jira 10 cusub oo Trinder's reagents ayaa diyaar ah.Ka mid ah kuwa cusub ee Trinder's, TOOS waa tan inta badan la isticmaalo.Si kastaba ha ahaatee, substrate gaar ah, tijaabinta fasalada kala duwan ee reagents-ga cusub ee Trinder ayaa lagama maarmaan u ah horumarinta nidaamka ogaanshaha ugu fiican.