Alaabta

MicroRNA (miRNA) biosensor aad xasaasi u ah oo u shaqeeya ruthenium oxide nanoparticle (RuO(2) NP) -polymerization bilaabay 3,3'-dimethoxybenzidine (DB) iyo meel dhigista miRNA-qaabaysan ee poly insulating (3,3'-dimethoxybenzidine) Filimka (PDB) ayaa lagu sifeeyay shaqadan.Biosensor-ku wuxuu ka samaysan yahay monolayer isku dhafan oo ah oligonucleotide probes qabashada (CPs) iyo 4-mercaptoaniline oo ku yaal koronto dahab ah.Isku-dhafka ka dib ruO(2) miRNA bartilmaameedka NP-tagged, isku dhafka DB/H(2)O (2) ee pH 5.0 0.10 M acetate buffer ayaa lagu dabaqay biosensor-ka.RuO(2) NP-yadu waxay u adeegaan sidii curiye/kataliyaha polymerization ee DB.Iyo xargaha miRNA-ga isku-dhafka ah iyo CP-yada bilaashka ah waxay u adeegaan qaab-qaabeeya, hagaya meel dhigista PDB.Qadarka PDB ee la xareeyay iyo awoodeeda dahaadhku waxay si toos ah ugu xidhan tahay fiirsashada miRNA bartilmaameedka ee xalka.Tijaabooyinka spectroscopic impedance electrochemical impedance spectroscopic tests waxay tuseen in xidhiidh toos ah oo wareejinta iska caabin-ururinta laga bilaabo 6 .0 fM ilaa 2.0 pM la gaadhay 60 min ka dib markii lagu beeray isku dhafka DB/H(2)O(2).Ma jirin isdhexgal u dhexeeya miRNA-ka-hor iyo miRNA qaan-gaar ah iyo is-dhexgal aad u yar oo ka dhex jira xubnaha qoyska miRNA ee xiriirka dhow la leh xitaa heerarka hal-saldhigga-isku-dhafan.Biosensor-ku-saleysan impedance wuxuu bixiyaa beddel soo jiidasho leh oo loogu talagalay muujinta muujinta miRNA waxaana laga yaabaa inay awood u siiso horumarinta nidaamka sifaynta miRNA ee la qaadan karo.