ONPG CAS: 369-07-3 98.0% Cadaan Min Dami -Budada Cad
| Lambarka buugaagta | XD90006 |
| CAS | 369-07-3 |
| Magaca Alaabta | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Qaanuunka molecular | C12H15NO8 |
| Miisaanka Molecular | 301.25 |
| Faahfaahinta Kaydinta | 2 ilaa 8 °C |
| Xeerka Tarifka La Iswaafajiyay | 29400000 |
Tilmaamaha Alaabta
| Daahirnimada (HPLC) | Min.98.0% |
| Muuqashada | Caddaan ilaa off - budo cad |
| Xalka(1% ee Biyaha) | Xalka cad, aan midab lahayn ama huruud yar |
| Waxyaalaha biyaha(Karl Fisher) | Max.0.5% |
| Wareegga indhaha ee gaarka ah [α]D20(c=1, H2O) | - 65.0 ° C ilaa -73.0 ° C |
Ka doodista tijaabada ONPG (tijaabada β-galactosidase)
Su'aalo badan ayaa la soo bandhigay dhawaan: 1. Maxaa ONPG loogu isticmaali karaa in lagu kala saaro halsano lactose daahday?2. Muxuu heerka qaranku u sheegayaa inay lama huraan tahay in 3% sodium chloride trisaccharide iron (ama trisaccharide iron) loo isticmaalo tijaabada ONPG?3. Vibrio parahaemolyticus, marka la samaynayo tijaabada OPNG, maxay tahay sababta toluene loogu daro hoos u dhac ku salaysan heerka?Waa maxay shaqadu?
Shirkadeena waxay dib u eegtay macluumaad badan, soo koobtay, waxayna kula wadaagtay hoos:
Mabda'a: Magaca Shiinaha ee ONPG waa o-nitrobenzene-β-D-galactopyranoside.ONPG waxaa lagu hydrolyzed karaa galactose iyo jaalaha o-nitrophenol (ONP) by β-galactosidase, sidaa darteed dhaqdhaqaaqa β-galactosidase waxaa lagu ogaan karaa isbeddelka midabka ee dhexdhexaad ah dhaqanka.
Laktoosku waa sonkor ay u baahan yihiin in la ogaado inta badan noolaha yaryar.Dheef-shiid kiimikaadkeedu wuxuu u baahan yahay laba enzymes, mid waa permease unug, lactose wuxuu galaa unugyada hoos yimaada ficilka permease;Midda kale waa β-galactosidase, taas oo hydrolyzes lactose galay galactose.Lactose iyo glucose.β-Galactosidase sidoo kale waxay si toos ah ugu dhaqmi kartaa ONPG si ay u nadiifiso galactose iyo o-nitrophenol (ONP).Waxa la samayn karaa 24 saac gudahood, xataa iyada oo lactose daahday.Sidaa darteed, waxay sharxaysaa natiijooyinka indho-indhaynta ee ka soo qaadashada dhaqanka 1 ee agar slant iyo ku tallaalida goobada buuxda 36°C 1-3h iyo 24h.Haddii β-galactosidase la soo saaro, waxay isu rogi doontaa huruud ah 1-3h, haddii aysan jirin ensaymkaas, ma beddeli doono midabka 24h.
Sida laga soo xigtay labada enzymes ee kor ku xusan, microorganisms waxaa loo qaybin karaa qaybaha soo socda:
1 lactose-fermenting (18-24 saacadood) bakteeriyada leh permease iyo β-galactosidase P + G +;
2 Khamiiriyeyaasha lactose-ka oo dib u dhacay (wax ka badan 24 saacadood) oo aan lahayn dhiig-bax la'aan laakiin leh galactosesidase: P-G+.
3 Khamiiriyeyaasha aan lactose-ka ahayn oo ay ka maqan yihiin permease iyo galactosidase labadaba: P- G-.
Tijaabada ONPG waxaa loo isticmaali karaa in lagu kala saaro bakteeriyada lactose-lag-fermenting (P-G+) iyo bakteeriyada lactose-ka ee aan khamiirin (PG-), sida:
1 Kala sooc fermentiyeyaasha lactose dambe (P-G+) iyo kuwa aan lactose-ka ahayn (P-G-).
(a) Citrobacter (+) iyo Salmonella arizonae (+) oo ka timid Salmonella (-).
(b) Escherichia coli (+) ka Shigella sonnei (-).
Waa maxay sababta ONPG loo sameeyay iyadoo la isticmaalayo dhaqan habeen dhaxe ah oo ku saabsan 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Shirkaddayadu waxay la tashatay macluumaad badan, laakiin ma jiro hadal cad.Kaliya website-ka FDA, waxaa ku qoran "Dhaqamada inoculate in lagu tijaabiyo dushooda sokorta birta saddex jibaaran slants iyo incubate 18 h at 37 ° C (ama heerkulka kale ee habboon, haddii loo baahdo) . Nafaqada (ama kuwa kale) agar slants ka kooban 1.0 % lactose sidoo kale waa la isticmaali karaa."Macnaheedu waa: Bakteeriyada tijaabada ayaa lagu tallaalay dhexdhexaadka birta trisaccharide waxaana lagu beeray 37°C ilaa 18h.Nafaqada agar slants (ama kuwa kale) dhexdhexaad ah oo ka kooban 1% lactose sidoo kale waa la aqbali karaa.Sidaa darteed, waxaa la qiyaasaa in dhexdhexaadinta birta trisaccharide ay ku jirto lactose.Ka dib korriinka habeenkii, bakteeriyadu waxay soo saartay β-galactosidase firfircoon oo wanaagsan.Isticmaalka bakteeriyada noocaas ah, ONPG waxaa si degdeg ah u burburin kara β-galactosidase.Dhacdada tijaabada ah ayaa dhakhso badan oo si fiican u muuqata.Intaa waxaa dheer, ku darida hoos u dhaca ee toluene iyo qubeyska biyaha ee 5 min dhamaantood waa si ay si buuxda u sii daayaan β-galactosidase.
