L-Methionine Cas: 63-68-3 99% budada crystalline cad
| Lambarka buugaagta | XD90287 |
| Magaca Alaabta | L-Methionine |
| CAS | 63-68-3 |
| Qaanuunka molecular | C5H11NO2S |
| Miisaanka Molecular | 149.21134 |
| Faahfaahinta Kaydinta | Ambient |
| Xeerka Tarifka La Iswaafajiyay | 29304010 |
Tilmaamaha Alaabta
| Qiimayn | 98% - 101.5% |
| Muuqashada | Kiristaalo cad ama budada crystalline |
| Gabagabo | |
| Aqoonsiga | Buuxiyay shuruudaha |
| pH | 5.6 - 6.1 |
| Khasaare xagga qallajinta | ≤ 0.3% |
| Sulfate (SO4) | ≤ 0.03% |
| Birta | ≤ 0.003% |
| Hadhaaga dabka | ≤ 0.4% |
| Chloride | ≤ 0.05% |
| Birta culus | ≤ 0.0015% |
| Nadiifnimada Chromotograhi | In aan ka badnayn 2.0% wadarta wasakhda ayaa la helaa |
| Wareegid gaar ah [α] D 2 5 | +22.4º ~ +24.7º |
Daraasadadeenii hore waxay daaha ka qaadeen doorkooda muhiimka ah ee marxaladaha hore iyo kuwa dambe ee caabuqa KSHV.Daraasaddan, waxaan ku sahaminnay isdhexgalka ORF45 anagoo adeegsanayna koox ka kooban unugyada difaaca jirka ee monoclonal.Marka lagu daro kinase-ka-baxsan ee hore loo aqoonsaday (ERK) iyo p90 ribosomal S6 kinase (RSK), borotiinada, waxaan helnay dhowr borotiinno kale oo la isku daray, oo ay ku jiraan kuwa caanka ah ee ~ 38 kDa iyo ~ 130 kDa.Mass spectrometry ayaa shaaca ka qaaday in borotiinka 38-kDa uu yahay fayras ORF33 iyo borotiinka 130-kDa uu yahay USP7 unug (ubiquitin-gaar ah protease 7).Waxaan u dhignay goobta ORF33-ku xidhidhsa kaarboxyl-terminal 19 amino acids ee aadka loo ilaaliyo (aa) ee ORF45 iyo USP7-ku xidhidhiyaha ujeedada heshiis la sheegay ee gobolka dhexe ee ORF45.Isticmaalka midabaynta immunofluorescence, waxaan ku aragnay midabaynta ORF45 oo leh ORF33 ama USP7 labadaba xaaladaha la kala qaaday iyo unugyada KSHV-cudud.Waxaa intaa dheer, waxaan ogaanay dib u dejinta ORF45-ku-tiirsanaanta ee qayb ka mid ah ORF33/USP7 oo laga soo bilaabo xudunta ilaa t cytoplasm.Waxaan ogaanay in ORF45 ay sababtay kororka ururinta borotiinka ORF33 ee la baabi'iyay haddii midkood ORF33- ama USP7-binding domain ee ORF45 la tirtiray.Intaa waxaa dheer, tirtirka terminus kaarboxyl ee la xafiday ee ORF45 ee genome-ka KSHV wuxuu si weyn hoos ugu dhigay heerka borotiinka ORF33 ee unugyada caabuqa ee KSHV wuxuuna baabi'iyay soo saarista fayraska farcanka ah.Isku soo wada duuboo, natiijadeenu kaliya ma muujinayso qaybo cusub oo ka mid ah ORF45 interactome, laakiin waxay sidoo kale muujinaysaa in isdhexgalka borotiinnadani ay muhiim u yihiin ku celcelinta KSHVKSHV ORF45 waa borotiinka shaqeynta badan oo looga baahan yahay KSHV lytic ku celcelinta, laakiin hababka saxda ah ee ORF45 ay u qabato hawlaheeda muhiimka ah ma cadda.Daraasadadeenii hore waxay daaha ka qaadeen in dhammaan borotiinka ORF45 ee unugyada ay ku jiraan dhismayaal miisaan-sare leh.Sidaa darteed waxaan raadinay inaan tilmaamno isdhexgalka ORF45 si aan u soo bandhigno aragtiyo ku aadan doorkeeda inta lagu jiro ku celcelinta lytic.Annaga oo adeegsanayna koox ka mid ah unugyada difaaca jirka ee monoclonal, waxaan sahaminnay ORF45 interactome-ka ee ku jira unugyada caabuqa ee KSHV.Waxaan aqoonsannay laba lammaane oo cusub oo isku xidha ORF45: borotiinka fayraska ORF33 iyo unugga ubiquitin-gaar ah protease 7 (USP7).Waxaan sidoo kale muujineynaa in isdhexgalka ka dhexeeya ORF45 iyo ORF33 ay muhiim u tahay soo saarista hufan ee qaybaha fayraska ee KSHV, iyadoo soo jeedinaysa in faragelinta la beegsanayo ee isdhexgalkan ay matali karto istaraatiijiyad cusub oo lagu joojinayo ku celcelinta KSHV.
