Alaabta

γ-Glutamyltranspeptidases (γ-GTs) waxay jebiyaan γ-glutamyl amide bond ee glutathione waxayna u wareejiyaan kooxda γ-glutamyl ee la sii daayay biyaha (hydrolysis) ama asiidhyada amino acids (transpeptidation).Enzymes-yadan meel walba leh waxay door muhiim ah ka ciyaaraan biosynthesis iyo hoos u dhigista glutathione, iyo sun-saarista xenobiotic.Halkan waxaan ku soo gudbineynaa qaabka 3Å xallinta crystal ee Bacillus licheniformis γ-GT (BlGT) iyo ka kakan ee l-Glu.Qaab dhismeedka raajada ayaa xaqiijinaya in BlGT ay iska leedahay N-terminal nucleophilic hydrolase superfamily waxayna daaha ka qaadaysaa in borotiinku leeyahay goob firfircoon oo furan oo la mid ah tii lagu soo warramey enzyme-ka isku midka ah ee Bacillus subtilis, laakiin ka duwan kuwa lagu arkay aadanaha γ-GT iyo loogu talagalay γ-GT-yada noolaha kale.Xogtu waxay soo jeedinaysaa in isku xidhka l-Glu ay keento dib u habaynta dabada C-terminal ee qaybta weyn ee BlGT oo ay ogolaato aqoonsiga koox ka mid ah haraaga aashitada kuwaas oo suurtogal ah in ay ku lug yeeshaan aqoonsiga birta.Doorka haraaga kuwan ku saabsan xasilloonida qaabaysan ee BlGT ayaa lagu bartay iyada oo lagu garto farsamaynta, firfircoonida enzymatic, kiimikooyinka iyo kulaylka ee afar nooc oo cusub oo Ala hal ah.Natiijooyinku waxay muujinayaan in beddelka Asp568 ee Ala ay saameynayso labadaba habaynta iyo xasilloonida qaab dhismeedka borotiinka.