Alaabta

N- (2-Hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline sodium milixda Isticmaalka iyo Isku-dhafka

Dhaqdhaqaaqa bayooloji: HDAOS waa sheeko cusub oo Trinder's reagent, derivative aniline aadka u-milmi kara, kaas oo si weyn loogu isticmaalo baadhista ogaanshaha iyo shaybaadhyada biochemical.

Codsiga: Reegent-ka cusub ee Trinder's waa mid aad u biyo-milmi kara, kaas oo si weyn loogu isticmaalo ogaanshaha ogaanshaha iyo shaybaarada biochemical.Waxaa jira faa'iidooyin dhowr ah oo ka sarreeya reageniyeyaasha chromogenic caadiga ah ee go'aaminta midabka midabka ee dhaqdhaqaaqa hydrogen peroxide.Dib-u-hawlgalayaasha Trinder-ka cusub waa kuwo deggan oo ku filan in loo isticmaalo xalka iyo hababka ogaanshaha dhuumaha tijaabada labadaba.Riwaayad cusub Trinder's reagent inta lagu guda jiro falcelinta isku xidhka oksaydhka leh 4-aminoantipyrine (4-AA) ama 3-methylbenzothiazolesulfonehydrazone (MBTH) iyadoo ay joogaan hydrogen peroxide iyo peroxidase Kiimikada, samaynta midabyo buluug ah ama midab buluug ah.Nuugista xuubka dheeha ee ku lammaan MBTH wuxuu ahaa 1.5-2 jeer ka sarreeya tan dheeha oo ay weheliso 4-AA;si kastaba ha ahaatee, xalka 4-AA wuxuu ahaa mid ka xasilloon marka loo eego xalka MBTH.Substrate-ka waxaa enzymatically oksaydhed by oxidase ay si ay u soo saaraan hydrogen peroxide.Fogaanta hydrogen peroxide waxay u dhigantaa xoojinta substrate-ka.Sidaa darteed, qadarka substrate-ka waxaa lagu go'aamin karaa horumarinta midabka falcelinta isku-xidhka oksaydhka.Glucose, aalkolada, acyl-CoA iyo kolestaroolka ayaa loo isticmaali karaa in lagu ogaado substrate-kaas oo ay weheliyaan novel Trinder's reagent iyo 4-AA.Waxaa jira 10 cusub oo Trinder's reagents ayaa diyaar ah.Ka mid ah kuwa cusub ee Trinder's, TOOS waa tan inta badan la isticmaalo.Si kastaba ha ahaatee, substrate gaar ah, tijaabinta fasalada kala duwan ee reagents-ga cusub ee Trinder ayaa lagama maarmaan u ah horumarinta nidaamka ogaanshaha ugu fiican.