Alaabta

Mid ka mid ah qodobbada ka hortagaya isticmaalka kiliinikada ee daawada dib-u-soo-nooleynta ee cudurrada carjawda xumaaday ayaa ah isha ku habboon unugyada.Chondrocytes, oo ah nooca unugyada kaliya ee carjawda, oo ku koray vitro marka la eego xaaladaha dhaqameed si loo ballaariyo tirooyinka unugyada waxay lumiyaan phenotype-kooda oo ay weheliso awoodda ay ku dhalin karaan unugyada cartilaginous hyaline.Daraasaddan waxaan ku go'aamineynaa in nidaamka 3D ee serum-iyo koritaanka-la'aanta ah ee nidaamka dhaqanka uu dib u soo celiyo awoodda chondrocytes ee la maro si ay u sameeyaan unugyada carjawda ee vitro, habka ku lug leh sox9.Bovine articular chondrocytes ayaa la marsiiyey laba jeer u oggolow balaadhinta lambarka unugga (P2) oo lagu beeray cufnaanta sare ee 3D kolaj-nooca-II-dahaarka leh ee warbaahinta ka kooban gulukooska sare ee lagu kabo insulin iyo dexamethasone (SF3D).Unugyada waxaa lagu sifeeyay ka dib ballaarinta monolayer iyo raacitaanka dhaqanka 3D iyadoo loo marayo cytometry socodka, muujinta hiddaha, iyo taariikhda.Isbeddelada hore ee dariiqyada gudbinta calaamadaynta inta lagu jiro redisferentiat ion ayaa lagu sifeeyay. Unugyada P2 waxay muujiyeen muuqaal antigen-u eg oo ah 99% CD44 (+) iyo 40% CD105 (+) iyo astaanta muujinta hidda-wadaha oo tilmaamaya unugyada interzone.P2 ee SF3D waxay muujisay hiddo-wadaha chondrogenic iyo matrix-ka-ka-baxsan ee urursan.Hoos u dhigista soo-dhoweynta insulinta (IR) ee leh HNMPA- (AM3) ama dariiqa PI-3/AKT kinase (oo ay ku shaqeyso daawaynta insulin) iyadoo Wortmannin ay hakisay isku-darka kolajka.HNMPA- (AM3) waxay hoos u dhigtay muujinta Col2, Col11, iyo hiddo-wadaha IR iyo sidoo kale Sox6 iyo -9.Isku-darka difaaca jirka iyo falanqaynta chromatin immunoprecipitation ee HNMPA- (AM3) -unugyada la daaweeyay waxay muujiyeen isku-xirnaanta isku-xireyaasha Sox6 iyo Med12 oo leh Sox9 laakiin waxay hoos u dhigtay Sox9-Col2a1 binding. Waxaan ku tilmaamaynaa hab dhaqameed cusub oo u oggolaanaya kordhinta tirada chondrocytes waxayna kor u qaadaa Samaynta unug carjawda oo kale ah oo hyaline ah oo qayb ahaan lagu xidho Sox9-Col2a1 insulin dhexdhexaadinaysa.Ku habboonaanta nudaha lagu sameeyay habkan ee loogu isticmaalo hagaajinta wadajirka ah waxay u baahan tahay in lagu baaro vivo.