Alaabta

Unugyada stem Mesenchymal (MSCs) ayaa lagu soo warramey inay yihiin il soo jiidasho leh oo loogu talagalay jiilka unugyada beta ee beddelka ah ee la tallaali karo.Khadka unug-unug ee dhallaanka mesenchymal embriyaha ee murine C3H10T1/2 ayaa loo aqoonsaday inuu yahay tusaale loogu talagalay MSCs, sababtoo ah kala soocitaankeeda faracyada badan.Ujeedada daraasaddan ayaa ahayd in la sahamiyo in unugyada C3H/10T1/2 ay awood u leeyihiin inay u kala soocaan unugyada soo saara insulin-ta (IPCs).Halkan, waxaan ku baarnay oo aan is barbar dhignay kala soocida in vitro ee jiirka MSCs iyo C3H10T1/2 unugyada IPCs.Ka dib markii unugyadu ay soo mareen kala-soocidda IPC, muujinta calaamadaha kala soocida waxaa lagu ogaadey immunocytochemistry, falcelinta silsiladda transcription-polymerase (RT-PCR), qiyaasta waqtiga-dhabta ah ee RT-PCR (qRT-PCR) iyo xoqitaanka Galbeedka.Soo-saarka insulinta waxaa lagu qiimeeyay baaritaanka difaaca jirka ee ku xiran enzyme-linked (ELISA).Intaa waxaa dheer, unugyadan la kala soocay ayaa lagu beeray jiirarka sonkorowga ee streptozotocin iyo hawlahooda bayooloji ayaa lagu tijaabiyay vivo.Daraasadani waxay soo sheegaysaa hab 2-heer ah si loo soo saaro IPC-yada unugyada C3H10T1/2.Marka la eego xaalado gaar ah oo kicin ah oo ah 7-8 maalmood, unugyada C3H10T1 / 2 waxay samaysteen jirka spheroid saddex-cabbir ah (SBs) iyo insulin qarsoodi ah, halka jiilka IPC-yada laga soo qaatay MSC-yada ay u baahan yihiin waqti dheer (in ka badan 2 toddobaad).Intaa waxaa dheer, IPC-yadan laga soo qaatay unugyada C3H10T1/2 ayaa lagu duray jiirarka macaanka waxayna hagaajiyaan gulukooska asalka ah, miisaanka jidhka waxayna muujiyeen tijaabada dulqaadka gulukoosta caadiga ah.Daraasadda hadda jirta waxay bixisay qaab fudud oo aamin ah in vitro si loogu sii baaro habka salka ku haya kala soocida IPC ee MSCs iyo daawaynta beddelka unugyada ee sonkorowga.