Alaabta

N7/O6 isdhexgalka isku xidhka dhulbaraha ee isku dhafan antitumor firfircoon Rh(2)(OAc)(4)(H(2)O)(2) (OAc(-) = CH(3)CO(2)(-)) oo leh jajabka DNA-da d (GpG) ayaa si aan mugdi lahayn loo go'aamiyay NMR spectroscopy.Go'aaminta raajada hore ee madax-ilaa-madaxa (HH) iyo madax-ilaa-dabada (HT) ee dirhodium tetraacetate oo leh 9-ethylguanine (9-EtGH) ayaa shaaca ka qaaday isku-xir aan horay loo arag N7 / O6 guanine nucleobases oo ku baahsan Rh -Rh bond.Maqnaanshaha protonation N7 ee pH hoose iyo korodhka muuqda ee aysidhka N1-H (pK(a) qiyaastii 5.7 marka la barbar dhigo 8.5 ee N7 kaliya ee ku xidhan platinum adducts), oo ay soo jeedisay pH ku tiirsanaanta titrations ee purine H8 (1). H NMR resonances Rh(2)(OAc)(2)(9-EtG)(2) iyo Rh(2)(OAc)(2-)[d(GpG)], waxay la socdaan bidentate N7/O6 ee nucleobases guanin.Qiimaha pK(a) ee lagu qiyaasay N1-H (de) protonation, laga soo bilaabo daraasaadka ku tiirsanaanta pH ee C6 iyo C2 (13) C NMR resonances ee Rh(2)(OAc)(2)(9-EtG)( 2) isomers, oo ku heshiiyey t tuubo ka soo jeeda H8 (1)H NMR titration resonance.Isbarbardhigga (13) C NMR resonances ee C6 iyo C2 ee dirhodium adducts Rh (2) (OAc) (2) (9-EtG) (2) iyo Rh (2) (OAc) (2) [d (GpG) )] oo leh dhawaaqyada u dhigma ee xadhkaha aan xidhnayn [ee pH 7.0 ee 9-EtGH iyo pH 8.0 ee d(GpG)], waxay muujinaysaa isbeddellada hoose ee Deltadelta qiyaastii 11.0 iyo 6.0 ppm ee C6 iyo C2, siday u kala horreeyaan;isbeddellada dambe waxay ka tarjumayaan saameynta O6 ku xiran tahay xarumaha dirhodium iyo kobcinta soo socota ee acidity N1-H.Heerarka ugu sarreeya ee H8/H8 ROE ee 2D ROESY NMR spectrum ee Rh(2)(OAc)(2)[d(GpG)] waxay muujinayaan qaabaynta madax-ilaa-madaxa ee saldhigyada guanine.Rh(2)(OAc)(2)[d(GpG)] dulucdu waxay soo bandhigaysaa laba israac oo midigta ah, HH1 R iyo HH2 R, iyadoo HH1 R ay saddex jeer ka badan tahay kan aan caadiga ahayn ee HH2 R. Sifaynta dhammaystiran ee labadaba Qoraallada ayaa shaaca ka qaaday dib-u-celinta 5'-G sonkorta sonkorta ilaa C3'-endo (N-nooca), sii-haynta C2'-endo (S-nooca) iswaafajinta 3'-G sonkorta sonkorta, iyo jihaynta lidka ku ah marka la eego glycosyl bonds.Astaamaha qaabdhismeedka ee lagu helay Rh(2)(OAc)(2))[d(GpG)] iyaddoo la adeegsanayo NMR spectroscopy waxay aad ula mid yihiin kuwa cis-[Pt(NH(3))(2))[d( GpG)] iyo daraasado qaabaynta molecular oo xaqiijinaya.