Alaabta

Kordhinta cadaadiska oksaydhka ee hoos yimaada xaaladaha hyperglycemic, iyada oo loo marayo is dhexgalka AGE-yada leh reseptors RAGE iyo iyada oo loo marayo kicinta calaamadaynta qoraalka dhexdhexaadinta interleukin, ayaa laga soo sheegay kansarka.Wax ka beddelka borotiinka ayaa lagu sahamiyay doorkooda horumarinta iyo horumarka kansarka iyo jawaabta autoantibody ee iyaga ka dhanka ah ayaa helaya xiisaha sida baaritaanka hore ee cudurka.Daraasadani waxay falanqeysay isbeddelada histone H1 marka la beddelo methylglyoxal (MG) iyo saamaynta ay ku leedahay auto-immunopathogenesis ee kansarka.Histone wax laga beddelay ayaa muujiyay wax ka beddelka haraaga udgoonka, bedeshay microenvironment tyrosine, isku xirka iskutallaabta intermolecular iyo jiilka AGEs.Waxay muujisay waji-xidhka balastarrada hydrophobic iyo isbeddelka hypsochromic ee gudaha ANS fluorescence gaar ah.MG histone H1 si gardar leh oksidhised taasoo keentay ururinta karbonyls fal-celineed.Cabbirrada CD-ga UV ee fog waxay tuseen kor-u-qaadista qaab-dhismeedka alfa-ka ee di-carbonyl iyo soo-saarka xaashida beta;iyo Denaturation Thermal (Tm) daraasaadka ayaa xaqiijiyay xasilloonida kulaylka ee histone wax laga beddelay.Falanqaynta FTIR waxay muujisay isbeddelka amide I band, jiilka kooxda karboxyethyl iyo gariirka N-CA ee taariikhda la beddelay.Falanqaynta LCMS waxay xaqiijisay samaynta Nε- (carboxyethyl) lysine iyo daraasadaha microscopic elektarooniga ah ayaa shaaca ka qaaday samaynta wadarta guud ee amorphous.Histone wax laga beddelay ayaa muujiyay isku-xidhka iskaashiga ee DNA-da oo beddelay.H1 oo la beddelay waxa ay keentay unugyada difaaca jirka sare ee bakaylaha iyo qaabka go'doonsan ee IgG ee sera bakaylaha oo lagu tallaalay H1 la beddelay ayaa muujisay xidhid gaar ah oo ay la socoto difaaca jirka ee falanqaynta Western Blot.IgG oo ka soocay sera bukaannada qaba kansarka sanbabada, kansarka qanjirka 'prostate', kansarka naasaha iyo kansarka madaxa iyo qoorta gobolka ayaa muujiyay aqoonsi wanaagsan oo loogu talagalay neo- epitopes on histone modified, taasoo ka tarjumaysa joogitaanka autoantibodies wareegaysa ee kansarka.Maaddaama ay warbixinadu soo jeedinayaan xiriirka ka dhexeeya dhidibka AGE-RAGE iyo kansarka, glycoxidation ee histone H1 iyo difaaceeda difaaca ayaa u xaaraya siyaabo lagu fahmo doorka borotiinnada nukliyeerka glycoxidative ee kansarka.