D-Luciferin Cas: 2591-17-5 99% ka baxsan cad ilaa budada jaalaha ah nbsp BeeETLE LUCIFERIN
Lambarka buugaagta | XD90248 |
Magaca Alaabta | D-Luciferin |
CAS | 2591-17-5 |
Qaanuunka molecular | C11H8N2O3S2 |
Miisaanka Molecular | 280.323 |
Faahfaahinta Kaydinta | -15 ilaa -20 °C |
Xeerka Tarifka La Iswaafajiyay | 29342080 |
Tilmaamaha Alaabta
Waxyaalaha biyaha | Max.2.0% |
Turbidity | ugu badnaan 2.0 NTU |
Wareegga indhaha ee gaarka ah | -36 ilaa -32 |
Muuqashada | Cad-cad ilaa budo jaale ah |
Daahirnimada HPLC | Ugu yaraan 99% |
Isku xidhka dabar goynta molar | daq 17900 L/(mol cm) |
Horudhac: D-luciferin waa substrate loogu talagalay adenosine triphosphate (ATP) falcelinta bioluminescence ku tiirsan.Mabda'a bioluminescence waa in luciferin ay oksidhid by luciferase joogitaanka ATP iyo oksijiinta.Qaabka falcelinta kiimikada waa sida soo socota: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Light.
Mechanism of action: Habka ficilka ee D-luciferin waa in marka la eego ficilka ATP iyo luciferase, luciferin (substrate) waa la oksijiye si ay u iftiimiso iftiinka.Marka luciferin uu ka badan yahay, tirada photon-yada la soo saaro waxay si togan ula xiriirtaa xoojinta luciferase.
Codsiga: D-luciferin waa substrate loogu talagalay adenosine triphosphate (ATP) falcelinta bioluminescence ku tiirsan.Dareen-celinta bioluminescence ee luciferin / luciferase waxaa badanaa loo isticmaalaa ogaanshaha ATP, dheef-shiid kiimikaad loo bedeli karo ATP (sida AMP, ADP, cAMP), iyo enzymes kuwaas oo dhalin kara ATP (sida creatine kinase, iwm.), falcelinta bioluminescence waa la isticmaali karaa.Lagu dabaqi karo ogaanshaha noocyo badan oo agab bayooloji ah.
Dhaqdhaqaaqa noolaha: D-Luciferin (Firefly luciferin) waa substrate caan ah oo loogu talagalay bioluminescence ee joogitaanka ATP si loogu isticmaalo sawir-qaadista bioluminescence-ku-saleysan luciferase iyo baaritaanka unugyada-ku-saleysan.
Daraasadaha gudaha ee vitro: D-luciferin waxay la falgashaa luciferase, ATP iyo oksijiin si ay u iftiimiso iftiinka, kaas oo lagu ogaado filim sawir leh oo xasaasi ah si loo arko unugyada fosfatase-ku-xidhan alkaline.
In vivo Studies: isticmaalka substrates D-luciferin iyo firefly luciferase waxay ilaalinaysaa isdhexgalka difaaca burada-hosting ee qaabka jiirka difaaca ee leh kansarka ugxan-sidaha, maadaama barnaamijyada bioluminescence ay aad ugu nugul yihiin koritaanka burooyinka marka loo eego tilmaamaha korodhka miisaanka jidhka.